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  • 1
    Electronic Resource
    Electronic Resource
    Formation of secretory granules in the Golgi apparatus of prolactin cells in the rat pituitary gland: A stereoscopic study (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 232 (1992), S. 169-179 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The mode of secretory granule formation in prolactin cells was analyzed in thin or thick sections of pituitary glands from non-lactating or lactating female as well as from male rats. In all these animals, the Golgi apparatus of prolacting cells consists of a continuous twisted ribbon-like structure that branches and anastomoses to form a hollow sphere located in the juxtanuclear area. The early signs of secretory granule formation are observed along the trans-aspect of the Golgi ribbon where progranules appear as focal distensions simultaneously occurring anywhere in the last trans thiamine pyrophosphatase (TPPase)-containing Golgi element. In the transmost Golgi saccule, such dilatations usually contain several nodular masses of electron opaque material which are separated from each other and from the saccular membrane by a less intensely stained material. While this transmost saccule becomes more fenestrated, its focal polynodular distensions seemingly yield polynodular tubular progranules which are initially closely apposed and usually parallel to the trans face of the Golgi ribbon. Subsequently, these progranules, which frequently show small membranous tubules or tubular networks attached to them, are seen some distance from the Golgi stacks and progresively transform into the more compact polymorphous granules characteristic of prolactin cells. These observations suggest that the polynodular tubular progranules arise by fragmentation of portions of the trans-Golgi elements rather than by fusion of small uninodular granules budding from the edges of a trans-Golgi saccule. Once the progranules have been liberated, the rest of the transmost Golgi element appears to break down into small residual networks, tubules, and vesicles. Thus, in prolactin cells as in other glandular cells, the whole transmost Golgi element would fragment during formation of prosecretory granules.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092320202
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  • 2
    Electronic Resource
    Electronic Resource
    Transport of casein submicelles and formation of secretion granules in the golgi apparatus of epithelial cells of the lactating mammary gland of the rat (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 235 (1993), S. 363-373 
    Details
    ISSN: 0003-276X
    Keywords: Prosecretory granules ; Trans-Golgi network ; Exocytosis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Lactating mammary glands fixed by perfusion with 5% glutaraldehyde subsequently were postfixed with potassium ferrocyanide reduced osmium or were treated with tannic acid. Stained thin sections were examined with the electron microscope and stereopairs were prepared. The distribution of casein submicelles was analyzed in the various components of the Golgi apparatus. The Golgi stacks were composed of five or six elements, all of which contained casein submicelles 20 nm in diameter. The cis-tubular network or cis-element, as well as the underlying three or four midsaccules, showed these casein submicelles either attached to their membrane or free in the lumen. The trans-most element of the stacks formed distended prosecretory granules in which both isolated or clustered casein submicelles were suspended in an electron-lucent fluid. These micellar aggregates increased in size and became progressively more compact to form spherical dense bodies or casein micelles, in which the individual 20 nm particles could easily be resolved. Casein micelles were seen in secretory granules in addition to a wispy material of low density. The numerous small spherical vesicles (80 nm or larger) seen on the cis, lateral, or trans aspects of the stacks did not appear to contain free casein submicelles. This raises questions regarding the role of these vesicles in the transport of casein macromolecules through the Golgi stacks. It was noticeable that in this Golgi apparatus a trans-Golgi network was limited to a few small residual tubules free from casein submicelles. It thus appears that the greater part of the trans-most Golgi element gives rise to the large prosecretory granules. After leaving the Golgi region and prior to exocytosis, the secretory granules often fuse to form larger granules before exocytosis. © 1993 Wiley-Liss, Inc.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092350305
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  • 3
    Electronic Resource
    Electronic Resource
    Striated anchoring fibrils-anchoring plaque complexes and their relation to hemidesmosomes of myoepithelial and secretory cells in mammary glands of lactating rats (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 237 (1993), S. 318-325 
    Details
    ISSN: 0003-276X
    Keywords: Basement membrane ; Striated anchoring fibrils ; Anchoring filaments ; Anchoring plaques ; Hemidesmosomes ; Myoepithelial cells ; Acinar cells ; Mammary glands ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Striated anchoring fibrils (SAF) are associated with the basement membrane underlying myoepithelial and acinar cells of mammary glands. Their proximal extremities are inserted in electron-dense areas of the lamina densa, the anchoring plaques seen facing the hemidesmosomes of both myoepithelial and acinar cells. In the case of myoepithelial cells, the hemidesmosomes show a thick cytoplasmic plaque applied to the basal plasma membrane in which cytoplasmic filaments are inserted. Facing this plaque but on the extracellular aspect and at a short distance of 5-10 nm, there is a thin layer of electron-dense nodular material called the subcell membrane plate, which is connected to the plasma membrane by short filamentous bridges. Between this subcell membrane plate and the anchoring plaque, there is an abundance of fine anchoring filaments crossing the lamina lucida. Such anchoring filaments are less abundant in the lamina lucida outside the hemidesmosomal areas. In the case of acinar cells, the cytoplasmic plaques of the hemidesmosomes are thin and the associated cytoplasmic filaments less conspicuous. No distinct subcell membrane plate is seen on the extracellular aspect of the plasma membrane facing the cytoplasmic plaque of the hemidesmosomes. However, in this area numerous anchoring filaments cross the lamina lucida between the plasma membrane and the SAF-anchoring plaque complex. The abundance, in these cells, of hemidesomomes and their association with SAF-anchoring plaque complexes seen in the basement membrane must constitute a strong attachment for both myoepithelial and acinar cells and bind them to the underlying collagen fibrils, thus preventing their detachment from the connective tissue during the contractions of myoepithelial cells during milk ejection. © 1993 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092370304
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  • 4
    Electronic Resource
    Electronic Resource
    Connections between the various elements of the Cis- and mid-compartments of the Golgi apparatus of early rat spermatids (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 240 (1994), S. 469-480 
    Details
    ISSN: 0003-276X
    Keywords: Cis-Golgi network (CGN) ; Intermediate compartment (IC) ; Golgi saccules and vesicles ; Spermatids ; Rat ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Background: The exact structural relationships of the saccules, membranous tubules, and vesicles that compose the cis- and midcompartments of the Golgi cortex of rat spermatids was investigated to determine the relationship of these elements to each other.Methods: Tissues fixed with glutaraldehyde and buffered in sodium cacodylate were examined with the electron microscope. Electron micrographs, including stereopairs, were analyzed to determine the three-dimensional organization of the Golgi elements.Results: The deeper layer of the Golgi cortex was composed of stacks of saccules connected to each other either by saccules or membranous tubules. The peripheral region of the Golgi cortex, located between the cisside of the stacks and a network of overlying ER cisternae contained numerous membranous tubules and vesicles of two class sizes: 50-100 nm vesicles and microvesicles 5-10 nm in diameter. The tubules formed tight networks, known as cis-elements or cis-Golgi networks (CGN), which were strictly parallel and next to the first or cis-saccule of the stack. The cis-elements were continuous with more loosely arranged peripheral tubules which formed elaborate, intertwined and interconnected networks. These peripheral tubules closely approximated the overlying ER cisternae in areas often showing fuzz-coated finger-like projections. Occasionally such peripheral tubules were continuous with ER cisternae. The saccules forming the stacks were continuous with membranous tubules which not only connected saccules of adjacent stacks, but also saccules of the same stack. These tubules were also connected with the tight tubular networks forming the cis-elements and the broad networks formed by the peripheral membranous tubules. Vesicles (50-100 nm) and microvesicles (5-10 nm) frequently formed aggregates in the peripheral Golgi region next to areas of ER membrane free of fuzz-coated projections. The microvesicles, embedded in a denser cytoplasmic matrix, had a more or less distinct delimiting membrane suggestive of their disintegration in this juxta-ER location. The 50-100 nm vesicles that were seen at the periphery of the vesicular aggregates appeared to form mainly from the membranous tubules of the Golgi cortex.Conclusions: Thus the saccules and membranous tubules of the spermatid's Golgi cortex formed a single continuous membranous system connected to ER cisternae. The vesicles, seemingly arising from the membranous tubules, appear to follow a retrograde pathway and undergo dissolution next to ER cisternae. © 1994 Wiley-Liss, Inc.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092400405
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  • 5
    Electronic Resource
    Electronic Resource
    Distribution of cell web-containing epithelial reticular cells in the rat thymus (1971)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 169 (1971), S. 613-625 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In sections of thymus stained with the tannic acid-phosphomolybdic acid-amido black (TPA) technique, the epithelial reticular cells can readily be identified by the well-stained tonofibrils in their cytoplasm. In the cortex, flattened epithelial reticular cells form a continuous layer on the inner surface of the capsule and along the interlobular septa. Within the cortex proper, stellate epithelial reticular cells are widely dispersed as a loose network. In the medulla, two zones, referred to as “outer” and “inner” medulla, are distinguished. The outer medulla, like the cortex, contains epithelial reticular cells, but these are more voluminous, are more richly provided with tonofibrils and form a denser network than in the cortex. In the inner medulla no epithelial reticular cells can be seen but instead connective tissue cells and fibers make up the supporting framework. A layer of flattened epithelial reticular cells demarcates the outer from the inner medulla. This layer of cells also extends along the outer surface of blood capillaries seen in the outer medulla and cortex. Around the larger blood vessels, this layer of epithelial reticular cells is separated from the vessel wall by a connective tissue perivascular space. Hence, the inner medulla is continuous with the perivascular spaces and, like them, is supported by connective tissue. Thus, the epithelial reticular cells constitute the supporting framework of the cortex and outer medulla and separate these regions from the connective tissue of the capsule, interlobular septa, blood vessels and inner medulla.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091690402
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  • 6
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of proteins utilized in the formation of outer dense fibers and fibrous sheath in rat spermatids: An electron microscope study (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 227 (1990), S. 447-457 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Affinity purified antibodies prepared against proteins isolated from fibrous sheath (FS) and outer dense fibers (ODF) were utilized in an immunocytochemical study of spermatids at various steps of spermiogenesis. This study, using the immunogold technique, was performed on sections of Epon or Lowicryl embedded tissues examined with the electron microscope. In the case of FS antibodies there was a selective immunoreactivity of the FS itself from step 10 onwards, but no reactivity over the plasma membrane associated FS anlagen. In addition there was a diffuse immunoreaction over the cytoplasmic matrix from step 9 until step 18 of spermiogenesis but no reactivity over the various types of dense bodies (e.g., granulated bodies, reticulated body, etc.) seen in the cytoplasm of these spermatids. In the case of ODF antibodies the ODF were immunolabeled throughout their development from step 11 onward. In addition to a diffuse immunoreactivity of the cytoplasmic matrix of spermatids from step 9 until step 18 of spermiogenesis, there was an immunolabeling of “granulated bodies.” These bodies appeared in relation to ER cisternae during steps 10-14, increased in number and size during steps 15-17 and decreased in number thereafter leaving only a few coarsely granulated bodies in the residual cytoplasm which detached from late step 19 spermatids. No other cytoplasmic structures were labeled with the ODF antibody-gold complexes. Thus the granulated bodies appeared to serve as a transitory storage site for some proteins destined to form ODF, a major cytoskeletal element of the tail of rat spermatozoa.
    Additional Material: 19 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092270408
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  • 7
    Electronic Resource
    Electronic Resource
    Modulation of the Golgi apparatus in Saccharomyces cerevisiae sec7 mutants as seen by three-dimensional electron microscopy (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 237 (1993), S. 441-452 
    Details
    ISSN: 0003-276X
    Keywords: Secretory pathway ; Secretory granules ; Glycoprotein transport ; Golgi saccules ; yeasts ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The three-dimensional configuration of the Golgi apparatus has been examined with the electron microscope in thick Golgi sections of Saccharomyces cerevisiae prepared from a wild-type strain and from sec7 mutants maintained for various periods of time at the nonpermissive temperature of 37°C and then returned to the permissive temperature of 24°C. Reduced osmium postfixation of glutaraldehyde fixed specimens stained intensely the content of Golgi elements and thus facilitated their three-dimensional characterization. In wild-type S. cerevisiae, the Golgi elements usually appeared as isolated networks of membranous tubules dispersed throughout the cytoplasm. Along such networks, distensions filled with stained material were similar in size to nearby secretory granules, suggesting that the latter formed by fragmentation of the Golgi elements. In sec7 mutants maintained at 37°C in low (0.1%) glucose medium, secretion granules progressively decreased in number and soon disappeared. Concomitantly the networks of Golgi tubules increased in size and complexity, lost their distensions, and then transformed into flattened sacules forming stacks of up to seven or eight saccules that were similar to the Golgi stacks seen in mammalian cells. However in contrast to the latter, connections between the saccules were evident and Golgi-associated small vesicles were generally absent. Following return to the permissive temperature (24°C), secretion granules reappeared, the Golgi stacks progressively decreased in size, and resumed their initial state of separated small tubular networks. Thus in sec7 mutant, grown at 37°C in low glucose medium, segregation of secretory granules is blocked. As a result, Golgi membranes accumulate to form a continuous system of stacked and interconnected saccules. © 1993 Wiley-Liss, Inc.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092370402
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  • 8
    Electronic Resource
    Electronic Resource
    Segregation of secretory material in all elements of the Golgi apparatus in principal epithellal cells of the rat seminal vesicle (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 232 (1992), S. 349-358 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: At the apex of the epithelial principal cells of the seminal vesicle, there appears to be two types of mature secretory granules, i. e., large and small. Both types of secretory granules showed an eccentric electron-dense spherical body with one pole attached to the delimiting membrane. The remainder of the large granule surrounding the eccentric body showed a granulofilamentous texture, whereas that of the small granule was electron lucent. The formation of these two types of granules was traced back to the various elements of the Golgi stacks. In the case of the large granules, the earliest stage of segregation of the precursor of the eccentric dense body was observed in distensions of the cis-element. Within distensions of all subjacent saccules, the dense bodies continued to be present but progressively increased in size while remaining attached to the saccular membrane. Following separation from the trans-face of the stack, the large prosecretory granules continued to increase in size by fusing with each other. The very large prosecretory granules, as they migrated toward the cell apex to become mature secretory granules, reduced in size prior to exocytosis. The small granules formed exclusively on the trans-aspect of the Golgi stacks and did not appear to fuse with each other. Observations on the formation of the large prosecretory granules within the Golgi apparatus and of the eccentric body in particular, which may be taken as a marker of the saccular membrane, were suggestive of a cis-trans migration and renewal of Golgi saccules.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092320304
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  • 9
    Electronic Resource
    Electronic Resource
    Modultion of the Golgi apparatus in stimulated and nonstimulated prolactin cells of female rats (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 235 (1993), S. 353-362 
    Details
    ISSN: 0003-276X
    Keywords: Progranules ; Tubular network ; Lactation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The three-dimensional structure of the Golgi apparatus and its compartments in prolactin cells has been examined in lactating rats in which secretion of prolactin was suppressed by removing the litter or stimulated by allowing the pups to suckle again. As soon as 2 hr after removal of the litter, large irregular progranules and numerous large pale vesicles accumulated in the trans-Golgi area together with vesicular or tubular fragments. The cis-tubular network was no longer recognizable on the cis-face of the Golgi ribbon; the saccules of the midcompartment were partitined by narrow fissures and also became perforated in register by numerous fenestrations of various sizes and irregular contours. The concomitant appearance of numerous vesicles in the cavities thus formed as well as in the surrounding cytoplasm indicated that they probably arose by the progressive cavitation and fragmentation of saccules of the mid compartment. Such a process, which reached a maximum between 4 and 6 hr after removal of the litter from the mother, was no longer observed at 8 and 12 hr, at which time intervals the Golgi apparatus was reduced in size with no cis-tubular elements and progranules on its trans-aspect and few vesicles in its surroundings. When mothers, separated from their litters for a period of 12 hr, were returned to their pups for 20 min, the cis-tubular network reappeared on the cis-aspect of the Golgi stacks and presumably formed by fusion of vesicles and anastomosed tubules located next to the cisternae of the rough endoplasmic reticulum. In addition, the structure of the midsaccules returned to the stimulated condition, and early progranules were again segregated within the trans-most saccules of the Golgi stack. Hence, the Golgi apparatus of prolactin cells was rapidly and deeply modified in the presence or absence of stimulation. © 1993 Wiley-Liss, Inc.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092350304
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  • 10
    Electronic Resource
    Electronic Resource
    Structure of the Golgi apparatus in stimulated and nonstimulated acinar cells of mammary glands of the rat (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 237 (1993), S. 308-317 
    Details
    ISSN: 0003-276X
    Keywords: Golgi apparatus ; Mammary gland ; Acinar cells ; Lactation ; Post-lactation ; Lactose ; Casein ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The structural features of the Golgi apparatus of acinar cells of mammary glands were examined with the electron microscope in 3 groups of rats: (1) in lactating female animals at 8 days postpartum, which served as controls; (2) in female rats sacrificed at various intervals from 2 to 30 hours following separation from their 8-day old pups; and (3) in females separated from their 8-day-old pups for a period of 12 hours and returned to their litters for durations of 1, 2, 4, and 8 horus. In animals of group 2, the Golgi stacks remained identical to that of controls between 2 and 8 hours. At 12 hours and later, the Golgi stacks decreased progressively in size, but the number of elements composing the stacks remained similar to that of lactating females and all contained casein submicelles. At 24 and 30 hours, typical secretory granules containing casein micelles disappeared from the trans aspect of the stacks. The earliest and most striking changes observed in the Golgi apparatus of the rats of group 2 took place at 12 hours. At this time, the prosecretory and secretory granules decreased considerably in volume and lost most of their electron-lucent content. This indicated that the delivery of small molecules, i.e., lactose and H2O, to these structures was soon altered following arrest of the sucking stimulus. In animals of group 3, the size of prosecretory and secretory granules and the amount of their electron-lucent content reverted to normal at 4 hours. Thus the influx of lactose and H2O into these structures appears to be rapidly restored after returning the pups to their mothers. The decrease in size of the Golgi stacks noted at 12, 18, and 24 hours following arrest of lactation (group 2), was accompanied by an increase in number of small vesicles that formed clusters next to the Golgi stacks and in “wells.” Thus in these regressing Golgi stacks, many of the associated small vesicles appear to arise by vesiculation of the saccules. © 1993 Wiley-Liss, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092370303
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