ISSN:
1573-5087
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract A pollen cDNA library has been constructed from Nicotiana tabacum and twenty pollen specific cDNA clones have been isolated by means of differential screening. The clones fall into ten homology groups on the basis of hybridisation data. Northern analysis was performed using the largest cDNA clone from each group and pollen specific messages were identified ranging between 1.2–4 kb. The predicted amino acid sequence of one cDNA clone, TP10, shows homology to the deduced amino acid sequences of two pollen specific pectate lyase genes identified in tomato while another clone, TP27 shows homology to a pollen specific polygalacturonase-like cDNA isolated from Oenothera organensis. The transcripts corresponding to both these cDNA clones are first detected late in pollen development, and are not detectable in vegetative tissues such as root, leaf, style or fruit. Genomic clones have been isolated from a tobacco genomic library using TP10 and TP27 as probes. The genomic clone for TP10 has been characterised and an open reading frame has been identified which shows 96% homology to the cDNA clone.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00024428
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