Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1990-1994  (2)
  • 1970-1974  (2)
  • 1
    Electronic Resource
    Electronic Resource
    The cell population in pancreatic islets of amphisbaenidae  -  a light and electron microscopic studyThis research was supported in part by United States Public Health Service Research grant AM-11407. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 167 (1970), S. 401-423 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A diversity of cell types have been found in the pancreatic islets of three species of Amphisbaenidae in a light and electron microscopic investigation. The A cells have been characterized as having phosphotungstic acid hematoxylin and orange G positive secretory granules which are electron opaque and 450-500 mμ, in diameter. The B cells possess aldehyde fuchsin reactive granules which are slightly larger than A granules, approximately 550 mμ, and have a variety of profiles in the electron microscope. The D cells are characterized as being fast green positive and argyrophilic in the light microscope, and in the electron microscope have small, 200-300 mμ, secretory granules with a core of moderate electron opacity. In D cells the Golgi apparatus is more highly developed than in A or B cells. Bipes islets contain a presumptive fourth cell type possessing large secretory granules with irregular profiles and focal internal densities. An additional cell type, found in Amphisbaena manni and Bipes, has small, electron opaque granules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091670404
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Cellular gene expression for calbidin-D28k in mouse kidney (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 227 (1990), S. 145-151 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Gene expression for calbindin-D28k, the 28,000 relative molecular mass vitamin D-dependent calcium-binding protein, was measured in cells of the murine nephron by in situ hybridization on tissue sections (hybridization cytochemistry). Radiolabeled (35S-UTP), single-stranded RNA complementary to calbinding-D28k-mRNA (probe RNA) was prepared from linearized cDNA template and used for the hybridizations. Autoradiography was carried out and cellular levels of hybridization signal (silver grains) were quantified. After corretion for background the concentration of silver grains was more than 350% greater in the distal tubule than in either the proximal tubule or the glomerulus. The relative cellular level of mRNA in the cytoplasm, as reflected in silver grains/cell, of the distal tubules with probe RNA was 3.4 times greater than that with control RNA. Cells of the distal tubule were the only apparent sites of specific hybridization with probe RNA. The presence of calbindin-D28k-mRNA in the distal tubule corresponded to the localizations of calbindin-D28k by immunocytochemistry.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092270202
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Occurrence and localization of calbindin-D28k in kidney and cerebellum of the slider turtle, Trachemys scripta (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 239 (1994), S. 185-190 
    Details
    ISSN: 0003-276X
    Keywords: Calbindin-D28k ; CaBP ; Turtle ; Immunocytochemistry ; Kidney ; Brain/cerebellum ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Backgrouns: Since its initial discovery in the avian intestine, calbindin-D28k has been reported to occur in various species and tissues. Although calbindin-D28k binds calcium ions in the physiologically relevant range of intracellular calcium, its functional role in the various cell types where it has been localized remains unknown.Methods: We examined the occurrence of calbindin-D28k in the brain and kidney of the testudine reptile, Trachemys scripta, by immunoblotting and immunocytochemistry using rabbit anti-sera directed against rat renal calbindin-D28k and chicken intestinal calbindin-D28k.Results: Immunoblotting revealed the presence of calbindin-D28k in the turtle tissues. A single immunoreactive band in the 28,000 relative molecular mass region was visualized in cerebellar and renal homogenates. Immunocytochemistry revealed reaction product for the presence of calbindin-D28k in the Purkinje cells of the cerebellum, and in the distal tubular cells of the nephron. Processes as well as the perikaryon of the Purkinje cell were immunoreactive.Conclusion: This study describes the occurrence and cellular localization of calbindin-D28k in a reptilian cerebellum, and confirms the phylogenetic distribution of renal calbindin-D28k to the oldest major reptilian group. © 1994 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092390209
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Emiocytosis of B granules from saurian pancreatic islets perifused in vitroThis research was supported by N.I.H. Postdoctoral Research Felloship AM52275, and in part by P.H.S. grants AM01226, AM03373 and AM06181. (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 138 (1973), S. 481-497 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Splenic islets of Anolis carolinensis incubated in an in vitro perifusion system with media containing a non-stimulatory concentration of glucose had the same ultrastructural appearance as islets removed from an intact animal. When horseradish peroxidase was included in this medium, no profiles suggestive of emiocytotic release of B granules were observed despite basal insulin secretion.With cytochalasin B and a high concentration of glucose, the B cells exhibited margination of their secretory granules and moderate degranulation, but rarely were profiles suggestive of emiocytosis seen. In the presence of cytochalasin B, glucose and the other insulin secretagogues used, the B cells were more degranulated than with only cytochalasin B and glucose present, but profiles indicative of emiocytosis were infrequently encountered. Emiocytosis of the B granules was demostrated convincingly with the inclusion of peroxidase and the above agents. Virtually all the B cells had at least one secretory granule fused with the limiting membrane and surrounded by the peroxidase, and some B cells exhibited multiple release of granules. From these observations it is concluded that emiocytosis plays a major role in the secretion of insulin from B cells of Anolis.The B cells from control perifusions, as well as from intact animals, did not contain a microfilamentous cell web. Granular or filamentous masses were not demonstrated in B cells from splenic islets perifused with cytochalasin B. The mechanism of emiocytotic release of insulin from the B cells of Anolis is discussed with reference to these observations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001380406
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...