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  • 1990-1994  (3)
  • 1970-1974  (1)
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  • 1
    ISSN: 1520-4804
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 55 (1990), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The H3 histamine receptor is a high-affinity receptor reported to mediate inhibition of CNS histidine decarboxylase activity and depolarization-induced histamine release. We have used (R)-α-[3H]methylhistamine, a specific, high-affinity agonist, to characterize ligand binding to this receptor. Saturation binding studies with rat brain membranes disclosed a single class of sites (KD= 0.68 nM; Bmax= 78 fmol/mg of protein). Competition binding assays also yielded an apparently single class of sites with a rank order of potency for ligands characteristic of an H3 histamine receptor: Nα-methylhistamine, (R)-α-methylhistamine 〉 histamine, thioperamide 〉 impromidine 〉 burimamide 〉 dimaprit. In contrast, kinetic studies disclosed two classes of sites, one with fast, the other with slow on-and-off rates. Density of (R)-α-[3H]methylhistamine binding followed the order: caudate, midbrain (thalamus and hippocampus), cortex 〉 hypothalamus 〉 brainstem 〉 cerebellum. These data are consistent with an H3 histamine receptor, distinct from H1 and H2 receptors, that occurs in two conformations with respect to agonist association and dissociation or with multiple H3 receptor subtypes that are at present pharmacologically undif-ferentiated.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The H3 receptor is a high-affinity histamine receptor that inhibits release of several neurotransmitters, including histamine. We have characterized H3 receptor binding in bovine brain and developed conditions for its solubilization. Particulate [3H]histamine binding showed an apparently single class of sites (KD= 4.6 nM; Bmax= 78 fmol/mg of protein). Of the detergents tested, digitonin at a detergent/protein ratio of 1:1 (wt/wt) yielded the greatest amount of solubilized receptors, typically 15–30% of particulate binding. Neither equilibrium binding of [3H]histamine to receptors (KD= 6.1 nM; Bmax= 92 fmol/mg of protein) nor the inhibitor profile was substantially altered by digitonin solubilization. However, solubilization did increase the rate of [3H]histamine association with and dissociation from the receptor. Size-exclusion chromatography indicated an apparent molecular weight of 220,000 for the solubilized receptor, and peak binding from this column retained its guanine nucleotide sensitivity. These last two observations are consistent with the solubilized receptor occurring in complex with a guanine nucleotide-binding protein.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 10 (1972), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: If there are gravity data and some geological information available from the region of an intermontane basin, then the volume of saturated sediments, volume of ground water available from storage, and the total volume of ground water can be determined from the gravitationally determined anomalous mass. The anomalous mass occurs because of a density contrast which exists between low density alluvium and high density bedrock which define the surface and subsurface boundaries of the basin. The gravity effect of the anomalous mass can be detected by a gravity survey of the basin after which it can be separated from other gravity effects by a regional-residual separation. The anomalous mass is uniquely determined by applying Gauss's theorem to the residual gravity map.A model of an intermontane basin is developed which relates the anomalous mass to the total volume of saturated sediments. The total volume of saturated sediments is determined from the anomalous mass, the density contrasts between unsaturated and saturated alluvium and bedrock, the area of the basin, and the water table depth.The volume of water available from storage and the total volume of water in the basin are determined from the volume of saturated sediments and the storage coefficient and porosity of the sediments.The method is illustrated by a case history from Avra Valley, Arizona. It is concluded that the major advantage of this method occurs when well data are not available, because it eliminates the need for arbitrary assumptions about subsurface basin geometry to determine the volume of saturated sediments.
    Type of Medium: Electronic Resource
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