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  • 1990-1994  (10)
  • 1965-1969  (2)
  • Cell & Developmental Biology  (11)
  • Calcification
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 3 (1969), S. 266-273 
    ISSN: 1432-0827
    Keywords: Calcification ; Arteries ; Membranes ; Elastic tissue
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Avec la modification d'une méthode de von Kossa nous avons macroscopiquement representé les dessins de calcification de la membrane élastique intérieure, ainsi que les grosses artères du bassin, des artères des extrémités inferieures, des artères du bras, de la rate et des reins. Dans les artères du type élastique, dans les A.a. ilicae communes et internae, se présentaient les dégénérations calcaires de la membrane élastique intérieure sous forme d'incrustations noires arrondies ou polygonales qui ont été formes en groupes. Elles étaient toujours présentes chez des enfants agés de plus de trois mois et chez des aux adultes. Dans les artères musculaires, se montraient les parties calcifiées de la membrane élastique intérieure en forme de «bandes de calcaire» noires et groupes en paires le long des bordures des fissures de la membrane. Dans le cas où existait une calcinose forcée, on pouvait montrer avec cette méthode tout le système de fissures de la mambrane élastique intérieure. Chez tous les morts âgés de 10 à 20 ans, nous avons trouvé des bandes de calcaire dans les artères musculaires des extrémités inferieures. Ces bandes de calcaire ont été constatées aussi dans des personnes plus âgées. Au point de la fusion des bandes de calcaire et des incrustations calcaire polygonales des dépôts calcaires en forme de feuille («feuilles de calcaire») se forment dans la membrane elastique. Les bandes de calcaire et les feuilles de calcaire représentent des points de cristallisation pour des dépôts calcaire granuleux. Ils se forment dans la plupart des cas sur le la côté externe tourné vers la media.
    Abstract: Zusammenfassung Mit einer modifizierten von Kossa-Methode wurden die Verkalkungsmuster der inneren elastischen Membran der großen Beckenarterien, der Arterien der unteren Extremität, der Oberarm-, Milz-und Nierenarterien makroskopisch dargestellt. In den Arterien vom elastischen Typ, i.e. in den Aa. ilicae communes et internae, erschienen die Verkalkungen der inneren elastischen Membran als schwarze rundliche oder polygonale Inkrustationen, die zu Gruppen angeordnet waren. Sie wurden häufig bereits bei Neugeborenen festgestellt. Bei Kindern, die älter waren als 3 Monate und bei Erwachsenen lagen sie stets vor. — In den muskulären Arterien traten die verkalkten Anteile der inneren elastischen Membran makroskopisch als schwarze paarweise angeordnete „Kalkbänder” entlang den Rändern vorgebildeter Membranspalten auf. Bei ausgeprägter Calcinose konnte mit der angewandten Methode das gesamte Spaltensystem der inneren elastischen Membran dargestellt werden. Kalkbänder wurden in den muskulären Arterien der unteren Extremität bei allen 10–20 Jahre alten Verstorbenen vorgefunden und waren auch in den nachfolgenden Altersstufen stets vorhanden. Beim Zusammenfluß von Kalkbändern und polygonalen Kalkinkrustationen entstehen in der inneren elastischen Membran folienartige Kalkablagerungen („Kalkfolien”). Die Kalkbänder und Kalkfolien stellen Kristallisationspunkte für körnige Kalkablagerungen dar, die zumeist an ihrer äußeren, der Media zugekehrten Seite entstehen.
    Notes: Abstract Calcification patterns of the internal elastic membrane of the main pelvic arteries, lower limb arteries, brachial, splenic and renal arteries were demonstrated grossly by a modified von Kossa technique. In the elastic segment in the common and internal iliac arteries, the membrane calcification appeared as groups of roundish or polygonal incrustations. They were found frequently in newborns, and were always present in infants of more than three months, as well as in adults. In the muscular arteries, the calcified parts of the internal elastic membrane appeared grossly as pairs of bands (“calcific bands”) along the edges of the pre-existing gaps in this membrane. When calcification was pronounced, the whole pattern of the membrane gaps could be demonstrated by the method used in this study. Calcific bands were found in the muscular arteries of the lower limbs in all 10–20 year-old subjects, and were always present in the older age groups. The confluence of calcific bands or polygonal membrane incrustations found in the iliac arteries lead to sheet-like membrane calcification. The calcific bands and sheets represent crystallizing points for grain-like calcific deposits, which appear later on the medial surface.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 22 (1992), S. 250-256 
    ISSN: 0886-1544
    Keywords: cytoskeletal localization ; signal transduction ; intermediate filaments ; rat basophilic leukemia cells ; translocation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Protein kinase C (PKC) isoforms are key mediators in hormone, growth factor, and neurotransmitter triggered pathways of cell activation (Nishizuka: Science 233:305-312, 1986; Nature 334:661-665, 1988). Stimulation of kinase activity by diacylglycerol and calcium often leads to translocation of PKC from the cytosol to a particulate fraction (Kraft and Anderson: Nature 301:621-623, 1983). The β isoform of PKC is translocated and degraded much more rapidly than the β isoform in phorbolester-stimulated rat basophilic leukemia (RBL) cells (Huang et al.: J. Biol. Chem. 264:4238-4243, 1989). We report here immunofluorescence evidence that the distributions of PKC α and β are strikingly different in antigen-activated RBL cells. PKC β associates with perinuclear filaments and filaments that extend from the perinuclear area to the cell periphery whereas PKC β concentrates in regions of the cell periphery. This distribution of PKC β is distinctly different from that of actin filaments and microtubules as determined by phalloidin staining and by anti-tubulin antibody labeling. In contrast, the staining patterns obtained with antibodies to PKC β and to the intermediate filament protein vimentin are almost identical, indicating that PKC β associates with vimentin filaments. These bundles of 100 Å filaments may provide docking sites for interactions of PKC β with its substrates and thus confer specificity to the actions of this isoform. © 1992 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
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  • 3
    ISSN: 0730-2312
    Keywords: stromal cells ; cytokines ; synergy ; high proliferative potential stem cell ; Dexter culture ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The intramedullary control of marrow cell production has been a difficult area to approach experimentally. The introduction by Dr. Dexter and colleagues of long-term stromal dependent culture systems for murine marrow and the adaptation of these systems to human marrow growth have allowed for in-vitro studies of stromal dependent hemopoiesis. Despite some controversy in this area, most studies appear to show that adherent murine or human stromal cells are capable of producing a relatively large number of hemopoietic growth factors including G-CSF, GM-CSF, CSF-1, IL-6 and, at least by PCR analysis, IL-3. Other work indicates that the most primitive hemopoietic cells which appear to be multifactor responsive adhere directly to these stromal cells presumably through mediation of various adherence proteins.An early acting, multilineage factor termed hemolymphopoietic growth factor-1 (HLGF-1) has been isolated from a murine stromal cell line and may be identical to the recently described ligand for the c-kit receptor. This may represent an important early survival/maintenance factor for stem cells in this system.Studies on primitive stem cells, especially the high proliferative potential colony forming cell (HPP-CFC), indicate that they are responsive to varying combinations of growth factors and that with increasing numbers of growth factors, as studied in serum-free systems, decreasing concentrations of the factors may be biologically active.These observations altogether suggest that intramedullary hemopoiesis may be regulated by the positioning of early multifactor responsive stem cells via adherent proteins in juxtaposition to synergistically acting combinations of grwoth factors attached to stromal cell surfaces or the extracellular matrix. In addition, selective production of different growth factors from different subsets of cells may create growth factor gradients and explain the spacial distribution of different cell types within the marrow cavity.
    Additional Material: 2 Ill.
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  • 4
    ISSN: 0730-2312
    Keywords: rat protein kinase C ; recombinant baculovirus ; antisera ; phorbol ester ; isoenzymes ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Expression of rat protein kinase C-δ (PKC-δ ) and PKC-ξ in insect cells using recombinant baculovirus resulted in the production of proteins with a molecular size of approximately 76 kD and 78 kD, respectively, as determined by immunoblotting with subtype-specific antisera. Although the PKC-ξ cDNA encoded for 592 amino acids, a 76 kD protein was also generated by in vitro transcription/translation. Extracts of cells expressing PKC-δ were able to bind phorbol ester to levels comparable to extracts of cells expressing PKC-α. No phorbol ester binding was, however, detected in insect cell extracts expressing PKC-ξ. However, similar levels of protein kinase activity were detected in lysates of cells expressing PKC-δ or PKC-ξ when protamine sulfate was used as exogenous substrate. Compared to protamine sulfate, both, myelin basic protein (MBP) or histone, were poor substrates for PKC-δ and PKC-ξ. In contrast to PKC-ξ, the PKC-δ enzyme activity phosphorylated MBP or histone in a phosphatidylserine-(PS)/diacylglycerol(DG)-dependent manner, albeit not to the same extent as PKC-α. Lack of stimulation of the enzyme activity of PKC-ξ by PS/DG, was confirmed by endogenous phosphorylation of insect cell proteins by PKC-ξ, whereas several insect cell proteins were phosphorylated by PKC-δ in a PS/DG-dependent manner, including a protein of 78 kD.Our data demonstrate that the 76 kD PKC-ξ, in contrast to PKC-δ, is unable to bind phorbol esters and displays a protein kinase activity that is independent of PS of PS/DG. In addition, staurosporine was about 2-4 order of magnitudes less effective in inhibiting the protein kinase activities of PKC-δ and PKC-δ when compared to PKC-ξ.
    Additional Material: 7 Ill.
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  • 5
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Non-radioactive detection of mRNA with in situ hybridization histochemistry has emerged as an important new technology for the study of gene expression. Quantitative in situ hybridization studies have generally relied upon counting of autoradiographic grains in the emulsion overlying cells containing hybridized, radioactively labeled probe. However, such high resolution studies require tedious grain counting over individual cells, frequently in addition to weeks of exposure to nuclear emulsion. The present report describes a quantitative, non-radioactive approach to the detection of a specific mRNA in the brain with the advantages of comparatively rapid tissue processing and computerized image analysis. The validity of this approach was tested by measuring the haloperidol-induced increase in the level of preproenkephalin mRNA in striatal sections of the rat brain using an RNA probe labeled with digoxigenin-11-UTP. Detection of probe hybridized to tissue sections was carried out enzymatically following complex formation with an antidigoxigenin-alkaline phosphatase conjugate. Using computerized image analysis, it was found that chronic treatment of rats with haloperidol resulted in a 50 ± 6% increase in striatal neuronal optical density, a value in good agreement with previous studies using low-resolution radioactive methods, showing a 30-80% increase in striatal preproenkephalin mRNA hybridization signal.
    Additional Material: 3 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 232 (1992), S. 612-619 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The first appearance and early development of two circumventricular organs, the area postrema (AP) and the subfornical organ (SFO), were investigated in human embryos and fetuses from the 4th to the 40th gestational weeks (GW). The AP appears very early in development, during the GW 10; its high vascularization can be seen from GW14, and differentiated neurons are observed from GW 16. The SFO is characterized by a late onset of development. It can first be distinguished at GW 17, but it does not attain cytological differentiation until the last weeks of gestation. It is suggested that the AP has important functions during fetal life, which are related to normal fetal weight and growth; in contrast the SFO, which is connected with drinking behavior and salt/water balance, seems to play a less essential role in early fetal life.
    Additional Material: 4 Ill.
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  • 7
    ISSN: 1040-452X
    Keywords: DNA hybridization ; Spermatozoa aneuploidy ; Aneuploidy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Human spermatozoa were studied with a nonradioactive in situ hybridization method. Using a chemically modified DNA probe and immunocytochemical reactions for visualization, it was possible to obtain hybridization signals in 31 of 32 semen samples. Positive hybridization reactions, depending on cell accessibility, varied from 40% to over 90% for the different samples. Using a chromosome 1-specific DNA probe, disomy for this chromosome was found in 0.67% of all accessible sperm cells.
    Additional Material: 3 Ill.
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  • 8
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Phase microscopic investigations of Kolmer-fixed, depigmented sections of the adult chicken retina have provided photomicrographic evidence of the existence of three different photoreceptors: single rods, single cones, and double cones. The rod extends the entire thickness of the visual cell layer and is characterized by a uniformly thick outer segment and a hyperboloid-containing inner segment which is devoid of an oil droplet. The single cone is the shortest element; it contains a red oil droplet. The double cone consists of two unequal members, a tall, slender chief cone and a broad accessory cone. The chief component contains a large yellow oil droplet, whereas the accessory cone houses a small, oval, yellowish-green droplet and a characteristically large, oval paraboloid. The rod hyperboloid and the accessory cone paraboloid contain glycogen. No colorless droplets have been observed. Owing to the close association between oil droplet color and cone type, three colored layers of oil droplets are formed within the thickness of the retina: a proximal row of red droplets (the short, single cones), an intermediate layer of yellowish-green droplets (the accessory cones), and a distal row of yellow droplets (the tall chief cones).
    Additional Material: 8 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 143 (1990), S. 222-225 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Normal human cells such as human diploid fibroblasts (HDF) have a finite pro-liferative lifespan in culture. Previous studies have shown that the limited lifespan phenotype is dominant in cell hybrids formed by fusion of HDF to at least 23 different kinds of immortal human cells. However, two independent studies reported that hybrid clones formed by the fusion of HDF to the HeLa variant D98 had unlimited division potential. Those results were potentially very important because they implied that a) there is a dominant mechanism for immortalization of human cells in addition to the well-documented recessive mechanism, and b) a dominant mechanism would lend itself to identification of the immortalizing gene. Consequently, we carried out more detailed studies of the behavior of D98 cells in hybrids. Our results indicate that the majority of D98 x HDF hybrid clones exhibit a clear-cut finite proliferative lifespan phenotype. In addition, these hybrid cell populations often give rise to an immortal focus of cells that can be seen to take over the population of mortal cells at the end of their lifespan. This phenomenon reconciles our data with the previous reports of immortal D98 x HDF hybrid clones and leads us to conclude that D98 cells do not express a dominant immortalizing gene.
    Additional Material: 2 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 158 (1994), S. 527-534 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In this study we have investigated whether cytokines, critical mediators of the immune response, might have a direct effect on the expression and/or function of the human hepatic asialoglycoprotein receptor (ASGPR). Binding and uptake of asialoglycoproteins by the human hepatoma cell line, HepG2, and by freshly isolated rat hepatocytes were inhibited by 50% after 3-6 hours and completely abolished following a 24 hour exposure to tumor necrosis factor (TNF) α, interferon (INF) α or γ, or interleukin-2 (IL-2). The loss of ASGPR binding activity mediated by IL-2 was reversible up to 4 hours of exposure and accompanied by the selective phosphorylatior, of the cell-surface receptor. Steady-state levels of total cellular ASGPR protein remained unchanged over the first 6 hours of IL-2 incubation but declined in a dose dependent manner thereafter. This down regulation of ASGPR expression was due to reduced synthesis as a result of reduced receptor transcript levels. No loss was detected, however, of cell surface-associated receptor protein even after 24 hours of IL-2 incubation, suggesting that cytokine induced phosphorvlation constitutes a mechanism to regulate receptor activity. © 1994 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
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