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  • 1990-1994  (2)
  • 1965-1969
  • Cell wall  (1)
  • Life and Medical Sciences  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Cell shaping and microtubules in developing mesophyll of wheat (Triticum aestivum L.) (1990)
    Springer
    Protoplasma 153 (1990), S. 141-148 
    Details
    ISSN: 1615-6102
    Keywords: Cell shaping ; Cell wall ; Immunofluorescence ; Mesophyll ; Microtubules ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Differentiated mesophyll cells ofTriticum aestivum (cv. Star) exhibit a lobed outline resembling tube-shaped balloons with almost regularly spaced constrictions. It was shown that these constrictions are probably the result of hoops of wall reinforcements laid down during early stages of cell expansion. It appears that these hoops prevent expansion in the corresponding regions and thus give rise to the peculiar cell shape. The comparatively thin cell walls of the bulges are uniformly reinforced after the lobed shape is established. By using immunofluorescence techniques a change in the pattern of cortical microtubule arrangement was observed which corresponded to the pattern of cell wall deposition. Discrete bands of microtubules were found beneath the sites of hoop reinforcement. These bands disintegrated during late stages of cell expansion with microtubules fanning out into the almost empty regions of the bulges.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01353998
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Physiological approach to heterologous human serum albumin production by Kluyveromyces lactis in chemostat culture (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 1297-1303 
    Details
    ISSN: 0749-503X
    Keywords: Yeast ; Kluyveromyces lactis ; recombinant ; phosphoglycerate kinase ; glycolysis ; heterologous protein ; rHSA ; chemostat culture ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Production of recombinant human serum albumin (rHSA) controlled by the constitutive promoter phosphoglycerate kinase was studied in Kluyveromyces lactis. It was governed by both cell concentration and glycolytic flow. The triggering of the fermentation metabolism by unfavourable culture conditions (pH, pO2, D) caused a decrease in the synthesis of the heterologous protein. The highest productivity (75 mg 1-1 per h) and rHSA concentration (62 mg 1-1) were obtained in chemostat culture with a dilution rate of 0·12 h-1 and with 38 g 1-1 dry weight.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320101006
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    Paper (German National Licenses)
    Fulltext
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