ISSN:
1432-2048
Keywords:
External spacer region
;
DNA curvature
;
Protein/DNA interactions
;
High-mobility-group protein
;
Zea (DNA)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The 3-kb external spacer from a maize (Zea mays L. cv. A619) nuclear rRNA gene unit which contains nine highly homologous 200-bp repeat elements was found to include a region with DNA-curvature properties. The centre of curvature was localized within repeats 5 and 6 using a circular permutation assay. A 60-bp-long subfragment of this region was found to interact with nuclear proteins, including high-mobility-group (HMG) proteins, and with the maize HMGa protein synthesized in Escherichia coli from a recombinant plasmid. The potential influence of the binding of the HMG proteins on the conformation of this subfragment was studied with a permutation assay based on a bending vector.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00195754
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