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Plasma reaction of group VI metal carbonyls (1992)

Suhr, H. ; Schmid, R. ; Stürmer, W.

Springer

Plasma chemistry and plasma processing 12 (1992), S. 147-159

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ISSN: 1572-8986

Keywords: Metal carbonyls ; PECVD ; chromium ; molybdenum ; tungsten

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology

Notes: Abstract The hexacarbonyl compounds of Cr, Mo, and W have been used as precursors in plasma-enhanced chemical vapor depositions (PECVD). They form films of good adherence on glass, ceramics, and a variety of polymers. The nature of the deposits depends very much on the composition of the gas, which forms the plasma. When pure argon is used, the resulting films contain considerable amounts of oxygen and carbon. Films deposited in hydrogen/argon mixtures consist of the metal and/or the carbide. With Ar/O2 mixtures, Mo(CO)6 and W(CO)6 are converted into films of MoO3 and WO3, respectively. When H2S/H2 mixtures are used as plasma gas, Mo(CO)6 yields films consisting of MoSx.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01447443

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On-line monitoring of monoclonal antibodies in animal cell culture using a grating coupler (1993)

Polzius, R. ; Bier, F. F. ; Bilitewski, U. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 42 (1993), S. 1287-1292

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ISSN: 0006-3592

Keywords: integrated optics ; grating coupler ; FIA ; on-line monitoring ; animal cell culture ; monoclonal antibody ; immunochemical sensor ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: A grating coupler was used for the on-line determination of monoclonal antibodies produced in perfused animal cell bioreactor. The device was connected with the culture vessel via a flow-injection analysis (FIA) system, which was controlled automatically. Specific antimouse lgG antibodies were immobilized on the surface of the sensor-chip. After injection of the sample, the binding of mouse lgG was observed in real time. The regeneration of the binding sites of the immobilized antibodies using an acidic solution allowed the on-line detection of produced monoclonal antibodies in the range of 10 to 150 μg/mL. In contrast to other techniques coupled to bioprocesses, the developed method represents a regenerable direct immunosensor. Results were compared with standard ELISA techniques (off-line) and a competitive immunochemical assay using the grating coupler (off-line). © 1993 John Wiley & Sons, Inc.

Additional Material: 7 Ill.