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  • 1990-1994  (2)
  • 1960-1964  (1)
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Material
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Dopamine-Neurotensin Interactions in Mesocortical Neurons (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 668 (1992), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1992.tb27338.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A Model for Morphogenesis (1960)
    [s.l.] : Nature Publishing Group
    Nature 186 (1960), S. 294-295 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THIS article describes a model which imitates certain aspects of morphogenesis and maintenance in a developing embryo. The model consists of a number of artificial 'cells' (constructed from radio parts) which can stimulate and inhibit one another by means of connexions which are made through a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/186294a0
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Sensitive mRNA detection using unfixed tissue: combined radioactive and non-radioactive in situ hybridization histochemistry (1992)
    Springer
    Histochemistry and cell biology 98 (1992), S. 39-49 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the present study some experimental parameters for in situ hybridization histochemistry (ISHH) have been analysed using35S-labelled and alkaline phosphatase-conjugated probes, in order to develop a reproducible double-labelling procedure. We have compared the total exclusion of tissue fixation with tissue sections fixed by immersion in formalin. In addition, the effect of dithiothreitol was assessed both when combining radiolabelled and non-radioactive probes on a single tissue section and when the probes were used separately. Hybridization of unfixed tissue resulted in stronger specific labelling and lower background both for radiolabelled and alkaline phosphatase-conjugated probes. No loss in tissue preservation was seen at the light microscopic level after hybridization of unfixed tissue. High concentrations (200 mM) of dithiothreitol strongly suppressed background when using35S-labelled probes, whereas in the non-radioactive procedure, alkaline phosphatase labelling could only be achieved with very low dithiothreitol concentrations (〈1 mM). This incompatibility led to a protocol using unfixed tissue sections and a sequential hybridization procedure, with the radiolabelled probe and high concentrations of dithiothreitol in the first step and the alkaline phosphatase-conjugated probe without dithiothreitol in the second step.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00716936
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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