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Immunological detection and localization of a calsequestrin-like protein in red beet and cucumber cells (1994)

Xing, T. ; Williams, Lorraine E. ; Nelson, S. J. ; [et al.]

Springer

Protoplasma 179 (1994), S. 158-165

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ISSN: 1615-6102

Keywords: Beta vulgaris ; Calcium binding ; Calsequestrin ; Cucumis sativus ; Endoplasmic reticulum

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Calsequestrin is a calcium binding protein present in the sarcoplasmic reticulum (SR) of animal muscle cells and is thought to be essential for the rapid uptake and release of Ca2+, and thus for the regulation of Ca2+-dependent cellular functions. Higher plant cells of red beet (Beta vulgaris L.) and cucumber (Cucumis sativus L.) contain a polypeptide of about Mr 55000 that cross-reacts with a monoclonal antibody raised against calsequestrin from rabbit skeletal muscle SR. In beet this protein changes its apparent molecular weight with pH as indicated in Western immunoblotting. Although this protein bound calcium it was not the dominant calcium-binding protein in red beet. Washing of beet root tissue leads to a slight increase of this polypeptide in microsomal fractions as indicated by immunoblotting. After immunoblotting to partially purified cell membrane fractions this polypeptide appeared to be predominantly associated with endoplasmic reticulum-enriched fractions. Immunogold labelling of ultrathin sections of cucumber hypocotyl using the anti-calsequestrin antibody showed that gold particles were very largely confined to the cytosol and often in close proximity to the ER. Clusters of up to nine gold particles were observed, often over small vesicular areas, as observed in some animal tissues. These results indicate that red beet and cucumber cells contain a protein which may be related to animal calsequestrin. It appears to be associated with the ER and could be involved in cellular calcium regulation.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01403954

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Dual capillary column supercritical fluid chromatography (1990)

Raynor, M. W. ; Moulder, R. ; Davies, I. L. ; [et al.]

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 13 (1990), S. 22-26

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ISSN: 0935-6304

Keywords: Supercritical fluid chromatography ; Dual capillary columns ; Polycyclic aromatic hydrocarbons ; Hop and beer bitter acids ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: A chromatograph equipped with one injection valve, two capillary columns with different selectivities, two flame ionization detectors and two electrometers is used to perform simultaneous dual capillary supercritical fluid chromatography. Two sets of retention times and indices are obtained for a mixture of polycyclic aromatic hydrocarbons, from one injection, enabling positive identification. The system is also used to rapidly select a stationary phase for separating hop and beer bittering acids.

Additional Material: 8 Ill.