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  • 1
    Electronic Resource
    Electronic Resource
    Differential expression of U5snRNA gene variants in maize (Zea mays) protoplasts (1993)
    Springer
    Plant molecular biology 21 (1993), S. 133-143 
    Details
    ISSN: 1573-5028
    Keywords: pre-mRNA splicing ; U5 small nuclear RNA ; gene variants ; differential expression ; upstream sequence element
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The small nuclear ribonucleoprotein particles U1, U2, U4/U6 and U5 participate in the removal of introns from pre-messenger RNAs in the nucleus. Three genes encoding U5snRNAs, the RNA moiety of U5snRNPs, have been isolated from maize. As in other plant UsnRNA gene families the three maize U5snRNA genes exhibit sequence variation. Two of the gene variants (MzU5.1 and MzU5.2) are clearly expressed after transfection into maize leaf protoplasts while the third gene variant (MzU5.3) is expressed at very low levels. These different levels of expression cannot be directly correlated with sequence changes in the highly conserved Upstream Sequence Element (USE) required for expression of Arabidopsis UsnRNA genes nor with differential stability of the U5snRNA transcripts. Further sequence elements may therefore have a role in regulating maize UsnRNA gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039624
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Efficient splicing of an AU-rich antisense intron sequence (1993)
    Springer
    Plant molecular biology 21 (1993), S. 205-211 
    Details
    ISSN: 1573-5028
    Keywords: AU-rich sequences ; intron ; pre-mRNA splicing ; reverse transcriptase-PCR ; splice site ; transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For successful splicing in dicot plants the only recognised intron requirements are 5′ and 3′ splice sites and AU-rich sequences. We have investigated further the importance of AU-rich elements by analyzing the splicing of an AU-rich antisense intron sequence. Activation of cryptic splice sites on either side of the AU-rich sequence permitted the efficient removal of this essentially non-intron sequence by splicing. This splicing event not only confirms the importance of AU-rich sequences but also has implications for the evolution of interrupted genes and the expression of heterologous genes in transgenic plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019937
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization and expression of U1snRNA genes from potato (1992)
    Springer
    Plant molecular biology 19 (1992), S. 959-971 
    Details
    ISSN: 1573-5028
    Keywords: gene expression ; gene variants ; pre-mRNA splicing ; pseudogenes ; U1 small nuclear RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract U1 small nuclear RNAs (U1snRNAs) occur in the nucleus of plants and animals where, complexed with several proteins in the form of U1 small nuclear ribonucleoprotein particles (U1snRNPs), they play an important role in precursor messenger RNA (pre-mRNA) splicing. Ten potato U1snRNA genes have been isolated on two genomic clones illustrating the clustering of this multigene family on the potato genome. Based on both the sequence of their coding regions and upstream regulatory elements, seven of the genes are potentially functional. The other three genes were pseudogenes with defective promoter or coding region sequences. Analysis of expression of individual cloned U1snRNA genes in transfected tobacco protoplasts was impossible due to the similarity of U1snRNA sequences in tobacco. However, by marking the coding regions with oligonucleotides or constructing chimaeric genes consisting of a potato U1snRNA promoter region and maize U5snRNA coding region, three of the U1 promoter regions were shown to be transcriptionally active.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040528
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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