ISSN:
1612-1112
Keywords:
Protein purification
;
HPLC of receptor
;
Calcium channel
;
1,4-Dihydropyridine receptor
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Functionally intact 1,4-dihydropyridine receptors have been isolated from digitonin-solubilized skeletal muscle membranes with the combined use of Wheatgerm Lectin-Sepharose affinity chromatography and ion-exchange HPLC. Lectin affinity chromatography was used for the purification of glycoproteins containing N-acetyl-glucosamines residues. Separation of functionally intact 1,4-dihydropyridine receptor/calcium channel complex from Mg2+-ATPase, one of the most abundant glycoproteins in skeletal muscle T-tubular membranes, was successfully performed by HPLC on a TSKgel DEAE-5PW column.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02269800
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