ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
2′-Deoxyuridine
;
Anomer separation
;
Isomer separation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A liquid chromatographic assay method was developed to separate 2′-deoxyuridine from its anomer and pentopyranosyl isomers. The influence of the pH of the mobile phase, the type of organic modifier and the column temperature on the chromatographic parameters was systematically investigated. Using a Hypersil C18 (5 μm) column at 10°C, the following mobile phase was found suitable: tetrahydrofuran −0.2 M potassium phosphate buffer pH 4.0-water (0.1∶5∶94.9, v/v).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02262250
Permalink