ISSN:
1432-0584
Keywords:
−α 4.2 Deletion
;
α-Thalassemia-2
;
−α G-Taichung
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary We sequenced part of the X boxes ofα-thalassemia-1 of Southeast Asia type (- -SEA) with−α 4.2,−α 3.7,−α G-Taichung, andα CSα. We found the X box of−α 3.7 belonged to the X box of α2 globin gene and the X box ofα csα contained X boxes of both al andα2 globin gene, whereas the X box of−α 4.2 and−α G-Taichung was a hybrid of X boxes of α2 and α1 globin gene. We also found there are two types of−α 4.2 deletion; type 1 is a common type of−α 4.2 deletion and type 2 is linkage to−α G-Taichung. We used a combination of two methods, the amplification refractory mutation system (ARMS) and the amplified created restriction sites (ACRS), to amplify the hybrids of X boxes specifically. The upstream primer for X box ofα2 globin gene was designed following the standard ARMS procedure to amplify the X segment of theα-globin gene. The downstream primer was designed according to the ACRS method to check the specificity of PCR products. Using this approach, we can diagnose the different types of−α 4.2 deletion. This kind of approach can also be used to amplify the specific region from the cluster of highly homologous genes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02215955
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