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The immunocytochemical localization of angiotensinogen in the rat ovary (1990)

Thomas, Walter G. ; Sernia, Conrad

Springer

Cell & tissue research 261 (1990), S. 367-373

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ISSN: 1432-0878

Keywords: Angiotensinogen ; Ovary ; Estrous cycle Renin-angiotensin system ; Atresia ; Immunocytochemistry ; Rat (Wistar)

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary The present study examined the presence and cellular distribution of angiotensinogen, the precursor to the angiotensin peptides, in the ovary of the normal cycling rat by immunocytochemistry. Angiotensinogen staining was present in the granulosa cells of maturing follicles and to a lesser extent in those undergoing atresia. Staining was not seen in the granulosa cells of primordial or early primary follicles. In maturing follicles intense staining for angiotensinogen was confined to the antral cell layers, cells of the cumulus oophorus and in the follicular fluid. Strong immunostaining was also seen in the germinal epithelium covering the ovary. Lighter angiotensinogen staining was observed in some parts of the cortical and medullary stroma and occasionally in corpora lutea. No variation in the intensity or pattern of angiotensinogen staining was observed throughout the estrous cycle. Comparison of the distribution of angiotensinogen with the previously described localization of renin, AII, angiotensin converting enzyme and AII receptors, suggests that there are a number of intra-ovarian sites at which AII could be produced.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00318679

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Comparison of two juvenile hormone radioimmunoassays (1993)

Goodman, Walter G. ; Huang, Z.-H. ; Robinson, Gene E. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Archives of Insect Biochemistry and Physiology 23 (1993), S. 147-152

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ISSN: 0739-4462

Keywords: honey bee ; Apis mellifera ; juvenile hormone ; radioimmunoassay ; hemolymph ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology

Notes: Juvenile hormone from the hemolymph of adult worker honey bees of known age and behavioral status was extracted and analyzed by two different radioimmunoassays in two independent laboratoies. The assays are different in hapten attachment, radiolabeled tracer, and the method by which bound and unbound hormone are separated. Despite these differences in the methods, hormone determinations were in excellent agreement at lower levels (0-50 ng/ml) but diverged as the hormone concentrations increased (〉 50 ng/ml). The relative changes are in good agreement, with a correlation coefficient of 0.97. © 1993 Wiley-Liss, Inc.

Additional Material: 1 Ill.