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  • 1990-1994  (3)
  • Asparagus  (1)
  • Transcription  (1)
  • apical meristems  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia japonica) by vitrification and subsequent high plant regeneration (1994)
    Springer
    Plant cell reports 13 (1994), S. 442-446 
    Details
    ISSN: 1432-203X
    Keywords: cryopreservation ; apical meristems ; vitrification ; wasabi (Wasabia japonica)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In vitro-grown apical meristems of wasabi (Wasabia japonica Matsumura) were successfully cryopreserved by vitrification. Excised apical meristems precultured on solidified M S medium containing 0.3M sucrose at 20°C for 1 day were loaded with a mixture of 2M glycerol and 0.4M sucrose for 20 min at 25°C. Cryoprotected meristems were then sufficiently dehydrated with a highly concentrated vitrification solution (designated PVS2) for 10 min at 25°C prior to a plunge into liquid nitrogen. After rapid warming, the meristems were expelled into 2 ml of 1.2M sucrose for 20 min and then plated on solidified culture medium. Successfully vitrified and warmed meristems remained green after plating, resumed growth within 3 days, and directly developed shoots within two weeks. The average rate of normal shoot formation amounted to about 80 to 90% in the cryopreserved meristems. This method was successfully applied to three other cultivars of wasabi. This vitrification procedure promises to become a routine method for cryopreserving meristems of wasabi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231963
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cryopreservation of in vitro-cultured multiple bud clusters of asparagus (Asparagus officinalis L. cv Hiroshimagreen (2n=30) by the techniques of vitrification (1992)
    Springer
    Plant cell reports 11 (1992), S. 433-437 
    Details
    ISSN: 1432-203X
    Keywords: Cryopreservation ; Vitrification ; Asparagus ; In vitro ; cultured bud clusters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A culture line of asparagus forming green bulbous structures consisting of numerous multiple bud clusters designated “bud clusters” was induced from a meristem culture of asparagus (Asparagus officinalis L.cv. Hiroshimagreen, 2n=30). Small cubic segments (2 mm3) cut from bud clusters were cryopreserved using three different cryogenic protocols. Only vitrification produced very high levels of shoot formation after cooling to −196°C. Segments were treated with a vitrification solution (PVS2) at 25°C for 45 min or at 0°C for 120 min prior to a direct plunge into liquid nitrogen. After rapid warming, the segments were expelled into Murashige and Skoog medium containing 1.2 M sucrose for 10 min and then plated on agar shoot outgrowth medium. The average rate of shoot formation of vitrified segments producing normal shoots was near 90% without any preculture and/or cold-acclimation treatment. Revived segments resumed growth within 3 days and developed about three shoots per segment. In vitro-cultured bud clusters appear promising as material for cryopreserving asparagus germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232685
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Dispersion of the structural organization of proplastid-nuclei in vitro changes the transcriptional activity of plastid genes (1991)
    Springer
    Protoplasma 163 (1991), S. 203-206 
    Details
    ISSN: 1615-6102
    Keywords: DNA-protein interaction ; Nicotiana tabacum ; Proplastid ; Proplastid-nuclei (nucleoids) ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have developed a novel assay system for analyzing the relationship between the structure and the transcriptional activity of the plastid-nuclei (plastid-nucleoids). The organization of morphologically intact proplastid-nuclei, isolated from tobacco cultured cells (line BY-2), was dispersed by treatment with NaCl at various concentrations and their transcriptional activities were examined by an assay of transcription in vitro and Southern hybridization. Disturbance of the structural organization of the proplastid-nuclei caused changes in both absolute and relative transcriptional activities of plastid genes, a result that suggests that the transcriptional activity of plastid genes may actually be regulated by structural changes in the plastid-nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01323345
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    Paper (German National Licenses)
    Fulltext
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