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  • 1990-1994  (2)
  • Asparagus  (1)
  • Transcription  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Cryopreservation of in vitro-cultured multiple bud clusters of asparagus (Asparagus officinalis L. cv Hiroshimagreen (2n=30) by the techniques of vitrification (1992)
    Springer
    Plant cell reports 11 (1992), S. 433-437 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Cryopreservation ; Vitrification ; Asparagus ; In vitro ; cultured bud clusters
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A culture line of asparagus forming green bulbous structures consisting of numerous multiple bud clusters designated “bud clusters” was induced from a meristem culture of asparagus (Asparagus officinalis L.cv. Hiroshimagreen, 2n=30). Small cubic segments (2 mm3) cut from bud clusters were cryopreserved using three different cryogenic protocols. Only vitrification produced very high levels of shoot formation after cooling to −196°C. Segments were treated with a vitrification solution (PVS2) at 25°C for 45 min or at 0°C for 120 min prior to a direct plunge into liquid nitrogen. After rapid warming, the segments were expelled into Murashige and Skoog medium containing 1.2 M sucrose for 10 min and then plated on agar shoot outgrowth medium. The average rate of shoot formation of vitrified segments producing normal shoots was near 90% without any preculture and/or cold-acclimation treatment. Revived segments resumed growth within 3 days and developed about three shoots per segment. In vitro-cultured bud clusters appear promising as material for cryopreserving asparagus germplasm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00232685
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  • 2
    Digitale Medien
    Digitale Medien
    Dispersion of the structural organization of proplastid-nuclei in vitro changes the transcriptional activity of plastid genes (1991)
    Springer
    Protoplasma 163 (1991), S. 203-206 
    Details
    ISSN: 1615-6102
    Schlagwort(e): DNA-protein interaction ; Nicotiana tabacum ; Proplastid ; Proplastid-nuclei (nucleoids) ; Transcription
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We have developed a novel assay system for analyzing the relationship between the structure and the transcriptional activity of the plastid-nuclei (plastid-nucleoids). The organization of morphologically intact proplastid-nuclei, isolated from tobacco cultured cells (line BY-2), was dispersed by treatment with NaCl at various concentrations and their transcriptional activities were examined by an assay of transcription in vitro and Southern hybridization. Disturbance of the structural organization of the proplastid-nuclei caused changes in both absolute and relative transcriptional activities of plastid genes, a result that suggests that the transcriptional activity of plastid genes may actually be regulated by structural changes in the plastid-nuclei.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01323345
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