ISSN:
1432-2242
Keywords:
Sugar beet
;
B. procumbens
;
Nematode resistance
;
Pulsed-field gel electrophoresis
;
DNA probes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have begun to apply techniques for the preparation and anaylsis of large DNA segments from sugar beet (Beta vulgaris) addition lines carrying a mitotically stable chromosome fragment from B. procumbens that confers monogenic resistance to the nematode Heterodera schachtii, with a view towards isolating the resistance gene. DNA probes specific for this chromosome fragment were selected, and various methods for cloning genome-specific fragments, including probes from megabase DNA separated in pulsed-field slab gels, are compared. Probes that display high homology to B. procumbens have been used for hybridization of a representative genomic library and for initial step in mapping the chromosome fragment via pulsed-field gel electrophoresis after restriction with infrequently cutting enzymes. Our data indicate that DNA molecules from the entire chomosome fragment can be separated from protoplast DNA lysates.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00226881
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