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  • 1
    Electronic Resource
    Electronic Resource
    Pigments of Rubrobacter radiotolerans (1994)
    Springer
    Archives of microbiology 162 (1994), S. 414-421 
    Details
    ISSN: 1432-072X
    Keywords: Key words     Carotenoid ; Bacterioruberin ; Red pigment ; Radiotolerance ; Rubrobacter radiotolerans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      The highly radioresistant Rubrobacter radiotolerans, contains red pigments. Since the pigments could not be extracted by usual methods, a new method was developed in which the pigments were extracted with organic solvents after addition of 10 N KOH to the intact cells, followed by neutralization. These pigments were also extracted after treatment with achromopeptidase, but not with lysozyme. The extracted pigments separated into two main spots by TLC (48.6% and 22.6%), and were confirmed to be carotenoids by chemical tests. The two major pigments had 13 conjugated double bonds as determined from the main maximum wavelength of the light absorption spectra. Their molecular weights were determined to be 740 and 722 by mass spectrometry. The mass spectra of their TMS-derivatives revealed that they contained four and three tertiary OH groups, respectively. Confirming their identical light and IR spectra, these pigments were determined to be bacterioruberin and monoanhydrobacterioruberin, respectively, the characteristic carotenoids of halophilic bacteria. The existence of these pigments in bacteria other than halobacteria provides interesting new evidence on the distribution of these compounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050159
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Pigments of Rubrobacter radiotolerans (1994)
    Springer
    Archives of microbiology 162 (1994), S. 414-421 
    Details
    ISSN: 1432-072X
    Keywords: Carotenoid ; Bacterioruberin ; Red pigment ; Radiotolerance ; Rubrobacter radiotolerans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The highly radioresistant Rubrobacter radiotolerans, contains red pigments. Since the pigments could not be extracted by usual methods, a new method was developed in which the pigments were extracted with organic solvents after addition of 10 N KOH to the intact cells, followed by neutralization. These pigments were also extracted after treatment with achromopeptidase, but not with lysozyme. The extracted pigments separated into two main spots by TLC (48.6% and 22.6%), and were confirmed to be carotenoids by chemical tests. The two major pigments had 13 conjugated double bonds as determined from the main maximum wavelength of the light absorption spectra. Their molecular weights were determined to be 740 and 722 by mass spectrometry. The mass spectra of their TMS-derivatives revealed that they contained four and three tertiary OH groups, respectively. Confirming their identical light and IR spectra, these pigments were determined to be bacterioruberin and monoanhydrobacterioruberin, respectively, the characteristic carotenoids of halophilic bacteria. The existence of these pigments in bacteria other than halobacteria provides interesting new evidence on the distribution of these compounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282106
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    GSMAC finite element method for unsteady incompressible Navier-Stokes equations at high reynolds numbers (1990)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 11 (1990), S. 479-499 
    Details
    ISSN: 0271-2091
    Keywords: GSMAC finite element method ; Incompressible Navier-Stokes solver ; High Reynolds number ; Driven cavity flow ; Vortex shedding behind a circular cylinder ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A new finite element technique is developed for predicting the velocity and the pressure in transient incompressible viscous fluid flows at high Reynolds numbers. The new method is based on the generalized and simplified marker-and-cell method (GSMAC) and has two characteristics: one is an application of the Bernoulli function and the implicit pressure solution algorithm to the explicit fractional time step method; the other is a high-order flux calculation to prevent the pressure field from oscillating. Two examples, driven cavity flows at high Reynolds numbers and vortex shedding behind a circular cylinder, are presented. Satisfactory agreement with experiment is demonstrated.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650110503
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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