ISSN:
1615-6102
Keywords:
NMR micro-imaging
;
Grape berry
;
Lipid
;
Water
;
Distribution
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The technique of nuclear magnetic resonance (NMR) micro-imaging has been applied to the study of water, lipid, and soluble carbohydrate distribution in intact grape berries. Conventional1H spin echo images reveal details of berry vascular structure, which can be enhanced by suitable choice of imaging parameters. Change in water distribution during drying can also be observed non-invasively. By means of chemical shift imaging methods, it is possible to image separately the distributions of water, lipid, and soluble carbohydrate (glucose and fructose) in selected berries, with an in-plane resolution of 60 μm. The technique demonstrated concentration of lipid (oil) in the seeds of intact, mature Shiraz grapes, in a relatively narrow zone in the endosperm, next to its interface with the inner layer of the seed coat, although the lipid zone did not extend entirely around the endosperm perimeter. Carbohydrate, consisting mainly of glucose and fructose in approximately equal proportions, was shown to be evenly distributed in the placental and pericarp tissues of mature grapes. In some cases magnetic field distortions due to the presence of air pockets in seeds of Chardonnay grapes and in seed traces of mature Sultana grapes were also present, leading to an artefactual appearance of localised high sugar in the chemical shift images of these berries.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01379006
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