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  • 1990-1994  (3)
  • Biochemistry  (1)
  • Fruit ripening  (1)
  • DNA laddering
  • Osteoarthritis
  • 1
    Electronic Resource
    Electronic Resource
    Accumulation of the β-subunit of polygalacturonase 1 in normal and mutant tomato fruit (1993)
    Springer
    Planta 191 (1993), S. 71-78 
    Details
    ISSN: 1432-2048
    Keywords: Fruit ripening ; Lycopersicon (fruit ripening) ; Mutant (tomato) ; Polygalacturonase (β-subunit)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyclonal antiserum raised against the native PG1 isoform of tomato fruit (Lycopersicon esculentum Mill.) polygalacturonase [poly(1,4-α-d-galacturonide) glycanohydrolase, EC 3.2.1.15] bound to each of the subunits of the protein and also to a range of other fruit proteins. Affinity purification was used to remove antibody molecules that bound to the native form of the PG2 isoform. The resulting serum bound to native PG1, denatured PG2 and β-subunits of PG1 but not to native PG2 or other fruit proteins. This anti-PG1 serum was used to monitor the occurrence of the PG1 β-subunit and PG2 in detergent extracts of tomato tissues. The β-subunit polypeptide was detected in pericarp but not locule tissue of fruit, including fruit of the rin and nor mutants. It increased in amount in the pericarp tissues from an early stage to the mature green stage, clearly prior to any appreciable accumulation of the PG2 subunit. The β-subunit polypeptide was not detected in stem or leaf tissues. A PG2-specific antiserum was used to study the interaction of PG2 with the isolated β-subunit. The PG2 isoform was bound to the β-subunit over a wide range of salt concentrations and pH; the interaction was independent of the presence of reducing agents. It is concluded that strong non-covalent forces are involved in the interaction. The results are consistent with a model in which the β-subunit is positioned in the cell wall structure and provides a specific binding site for the active PG2 subunit when this is synthesised during ripening.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00240897
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Osteoarthritis: a review of old myths and current concepts (1990)
    Springer
    Skeletal radiology 19 (1990), S. 327-333 
    Details
    ISSN: 1432-2161
    Keywords: Osteoarthritis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Radiology is dependent on an accurate understanding of the pathological process. This review of primary osteoarthritis identifies eight areas in which widely held concepts are either demonstrably false or fall short of proof. In some, the concepts have been disproved by the gradual accumulation of evidence. In others, the error is due to radiological misinterpretation while in a third group, the evidence is not in dispute, but the logical framework used in its assessment is flawed. Awareness of these deficiencies simplifies radiological interpretation and clarifies research objectives.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193085
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Conformational preferences of XONO2 systems (X = H, F, Cl, CH3) from ab initio techniques (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 12 (1991), S. 565-574 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: Ab initio SCF and MP2 methods have been used to calculate the geometries and relative energies of both planar and nonplanar rotamers of several nitrates with a DZP basis set. The planar arrangement of atoms is found to be the lower energy configuration in all cases. The interconversion between rotamers is analyzed by partitioning the contributions to the total SCF energy in a variety of ways.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540120506
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    Paper (German National Licenses)
    Fulltext
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