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  • 1990-1994  (2)
  • Cerium-based-enzymology  (1)
  • Chemistry  (1)
  • Polymer and Materials Science
  • 1
    Electronic Resource
    Electronic Resource
    Ultracytochemical demonstration of ouabain-sensitive, K+-dependent,p-nitrophenylphosphatase (Na-K ATPase) activity in cat facial nerve (1994)
    Springer
    European archives of oto-rhino-laryngology and head & neck 251 (1994), S. 238-240 
    Details
    ISSN: 1434-4726
    Keywords: Potassium-dependent ; p-nitrophenylphosphatase ; Facial nerve ; Cerium-based-enzymology ; Ultracytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Ouabain-sensitive, K+-dependent,p-nitrophenylphosphatase (K-NPPase) is the second dephosphorylative property of the Na-K ATPase complex. Localization of its activity in the horizontal portion of the facial nerve in 11 normal cats was studied ultracytochemically using a cerium-based method. The fine granular reaction product of the K-NPPase activity was observed on the cytoplasmic side of the axolemma of the axon cylinder. Enzyme activity was also detected on the cytoplasmic side of the plasma membrane of Schmidt-Lanterman incisures and nodes of Ranvier. No reaction product was detected on the periaxonal and outermost plasma membrane of Schwann cells and in the myelin sheath. In control tissue samples, enzyme activity was almost completely inhibited by 10 mM ouabain, and no reaction was noted in medium without K+. The present findings indicate that localization of Na-K ATPase in the cat facial nerve simulates that of other peripheral and cranial nerves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00628431
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    On-line combination of supercritical-fluid chromatography with fast-atom bombardment mass spectrometry (1990)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 4 (1990), S. 381-383 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Studies on combined supercritical-fluid chromatography (SFC) using a packed column, with fast-atom bombardment mass spectrometry (FAB-MS) are described. The interfacing was successfully carried out by simply coupling a frit interface between the liquid chromatograph (LC) and the mass spectrometer. The interface was equipped with a pneumatic splitter and post-column addition device delivering FAB matrix material to a release valve which maintained a constant back-pressure at the column outlet. Using a 1% glycerol solution as a matrix compound in the release valve, the SFC/FAB-MS combination operates as well as a normal LC/MS instrument. The performance was demonstrated with a mixture of α-, β- and γ-tocopherol and Triton X-100 (poly(ethylene glycol) p-isooctylphenyl ether).
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290041007
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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