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  • 1990-1994  (5)
  • Computational Chemistry and Molecular Modeling  (2)
  • enoyl-ACP reductase  (2)
  • Depression
  • Polymer and Materials Science
  • 1
    Electronic Resource
    Electronic Resource
    The use of a hybrid genetic system to study the functional relationship between prokaryotic and plant multi-enzyme fatty acid synthetase complexes (1994)
    Springer
    Plant molecular biology 25 (1994), S. 771-790 
    Details
    ISSN: 1573-5028
    Keywords: Escherichia coli envM gene ; enoyl-ACP reductase ; fatty acid synthetase ; gene replacement ; diazaborine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fatty acid synthesis in bacteria and plants is catalysed by a multi-enzyme fatty acid synthetase complex (FAS II) which consists of separate monofunctional polypeptides. Here we present a comparative molecular genetic and biochemical study of the enoyl-ACP reductase FAS components of plant and bacterial origin. The putative bacterial enoyl-ACP reductase gene (envM) was identified on the basis of amino acid sequence similarities with the recently cloned plant enoyl-ACP reductase. Subsequently, it was unambiguously demonstrated by overexpression studies that theenvM gene encodes the bacterial enoyl-ACP reductase. An anti-bacterial agent called diazaborine was shown to be a specific inhibitor of the bacterial enoyl-ACP reductase, whereas the plant enzyme was insensitive to this synthetic antibiotic. The close functional relationship between the plant and bacterial enoyl-ACP reductases was inferred from genetic complementation of anenvM mutant ofEscherichia coli. Ultimately,envM gene-replacement studies, facilitated by the use of diazaborine, demonstrated for the first time that a single component of the plant FAS system can functionally replace its counterpart within the bacterial multienzyme complex. Finally, lipid analysis of recombinantE. coli strains with the hybrid FAS system unexpectedly revealed that enoyl-ACP reductase catalyses a rate-limiting step in the elongation of unsaturated fatty acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028873
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    cDNA cloning and expression of Brassica napus enoyl-acyl carrier protein reductase in Escherichia coli (1991)
    Springer
    Plant molecular biology 17 (1991), S. 895-909 
    Details
    ISSN: 1573-5028
    Keywords: acyl carrier protein ; Brassica napus ; enoyl-ACP reductase ; fatty acid synthesis ; seed development ; nuclear-encoded chloroplast proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The onset of storage lipid biosynthesis during seed development in the oilseed crop Brassica napus (rape seed) coincides with a drastic qualitative and quantitative change in fatty acid composition. During this phase of storage lipid biosynthesis, the enzyme activities of the individual components of the fatty acid synthase system increase rapidly. We describe a rapid and simple purification procedure for the plastidlocalized NADH-dependent enoyl-acyl carrier protein reductase from developing B. napus seed, based on its affinity towards the acyl carrier protein (ACP). The purified protein was N-terminally sequenced and used to raise a potent antibody preparation. Immuno-screening of a seed-specific λgt11 cDNA expression library resulted in the isolation of enoyl-ACP reductase cDNA clones. DNA sequence analysis of an apparently full-length cDNA clone revealed that the enoyl-ACP reductase mRNA is translated into a precursor protein with a putative 73 amino acid leader sequence which is removed during the translocation of the protein through the plastid membrane. Expression studies in Escherichia coli demonstrated that the full-length cDNA clone encodes the authentic B. napus NADH-dependent enoyl-ACP reductase. Characterization of the enoyl-ACP reductase genes by Southern blotting shows that the allo-tetraploid B. napus contains two pairs of related enoyl-ACP reductase genes derived from the two distinct genes found in both its ancestors, Brassica oleracea and B. campestris. Northern blot analysis of enoyl-ACP reductase mRNA steady-state levels during seed development suggests that the increase in enzyme activity during the phase of storage lipid accumulation is regulated at the level of gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037070
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Amplitude squared squeezed states in the Fock-Bargmann space (1993)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 46 (1993), S. 515-518 
    Details
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Squeezed states are normally studied in configuration space or using the Glauber formalism for the coherent states. Here we show that the use of the Fock-Bargmann space can lead to more general solutions, especially in the case of squeezed states associated with the square of field amplitude. The relations that we obtain are completely general and several well-known, approximate results can be obtained as the limit cases of our approach.© 1993 John Wiley & Sons, Inc.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/qua.560460402
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  • 4
    Electronic Resource
    Electronic Resource
    Electrochemical study of iproniazid and its analytical determination by differential pulse polarography (1994)
    Weinheim : Wiley-Blackwell
    Electroanalysis 6 (1994), S. 491-496 
    Details
    ISSN: 1040-0397
    Keywords: Iproniazid ; Differential pulse polarography ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Iproniazid was found to be irreversibiy reduced in two steps, involving the exchange of two electrons and two protons each, by using tast polarography, normal pulse polarography (NPP), and cyclic voltammetry (CV). The rate constants of these two steps were found to be 5.8 × 10-6 and 4.2 × 10-6 and 4.2 × 10-6 cm s-1, respectively, and their corresponding diffusion coefficients 4.5 × 10-5 and 8.3 × 10-5 cm2 s-1, Also, α, the coefficient of cathodic transfer, was found to be 0.20 to 0.25 for the first peak and 0.17 to 0.15 for the second. On the basis of the electrochemical study performed, a differential pulse polarographic method for the determination of iproniazid over the concentration range of 5 × 10-7 M to 1 × 10-4 M was developed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elan.1140060522
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  • 5
    Electronic Resource
    Electronic Resource
    Franck-Condon factors and squeezed states (1994)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 52 (1994), S. 261-264 
    Details
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Similarities between the Franck-Condon effect and the concept of Squeezed States have been advanced as a conjecture in the past, or noticed as a marginal fact, without giving a full explanation of how these two important concepts are connected. In this work we outline a unifying formulation that contains as special cases both phenomena. The power of the new approach is shown in a general formula from which all particular cases can be derived. © 1994 John Wiley & Sons, Inc.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/qua.560520827
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    Paper (German National Licenses)
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