ISSN:
1432-2242
Keywords:
Cytoplasmic male sterility
;
Brassica oxyrrhina
;
B. campestris
;
B. juncea
;
Alien gene transfer
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Synthetic alloploid Brassica oxyrrhina (2n = 18, OO) x B. campestris (2n = 20, AA) was repeatedly backcrossed with B. campestris to place B. campestris nucleus in the cytoplasm of B. oxyrrhina. Alloplasmic plants, obtained in BC5 generation, were stably male sterile but mildly chlorotic during initial development. Synthetic alloploid B. oxyrrhina-campestris was also hybridized with B. juncea to transfer B. oxyrrhina cytoplasm. Segregation for green and chlorotic plants was observed in BC1 and BC2 generations. By selection, however, normal green male sterile B. juncea was obtained in BC3. Pollen abortion in both B. campestris and B. juncea is post-meiotic.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00225965
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