ISSN:
1617-4623
Keywords:
Specialized transduction
;
bio gene
;
Illegitimate recombination
;
REP sequence
;
DNA gyrase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary To examine the mechanism of recombination involved in the formation of specialized transducing phage during the induction of bacteriophage λ we have determined the nucleotide sequences of the recombination junctions of λbio phages. The results indicate that abnormal excision takes place at many sites on both bacterial and phage genomes and that the recombination sites have short regions of homology (5–14 bp). Some of the sequences of the recombination sites were similar to the consensus sequences of DNA gyrase-cleavage sites and repetitive extragenic palindromic (REP) sequences. These results showed that abnormal excision is a type of illegitimate recombination. The possible involvement of DNA gyrase in this recombination is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00290651
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