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  • 1990-1994  (2)
  • Environmental conditions  (1)
  • Male gamete (angiosperm)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 89 (1994), S. 814-817 
    ISSN: 1432-2242
    Keywords: Cytoplasmic inheritance ; Environmental conditions ; Nicotiana tabacum ; Sperm cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In flowers grown at warm temperatures in environmental chambers and at cooler temperatures in the greenhouse, eight parameters of the sperm-cell organization of Nicotiana tabacum were examined during sperm cell maturation using serial ultrathin sectioning, transmission electron microscopy and quantitative cytology. Despite employing the same seed source, and similar soil and nutrient conditions, the surface area and volume of the cell, the nucleus and the chondriome were larger in flowers grown in growth chambers under warmer controlled conditions, whereas the number of plastids appeared to be the same, or slightly higher, in flowers grown under cooler greenhouse conditions. These results suggest that environmental conditions may influence the quantity of cytoplasmic organelles, including mitochondria and plastids, thus potentially influencing the likelihood of male cytoplasmic inheritance.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1615-6102
    Keywords: Cytoplasmic inheritance ; Male cytoplasmic diminution ; Male gamete (angiosperm) ; Male germ unit ; Nicotiana ; Tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The organization of the sperm cells and vegetative nucleus (male germ unit) ofNicotiana tabacum was examined 18 h after semivivo pollination using transmission electron microscopy, computerassisted serial section reconstruction and quantitative cytology. Based on a measurement of 11 cellular parameters in nine reconstructed sperm cell pairs, there are no statistically significant differences between the two cells. The Svn is characterized by a strapshaped cytoplasmic extension that is physically associated with the surface of the vegetative nucleus. The nucleus is located adjacent to the sperm crosswall, with sperm organelles being distributed between the nucleus and the extension. The Sua is a tapered cell with cytoplasmic areas at both poles and deep axial invaginations near the crosswall. This cell has a centrally-located nucleus and a largely polar distribution of organelles. Three mechanisms for cytoplasmic diminution were observed that appear to contribute actively to the loss of cytoplasmic volume and organelles: (1) enucleated cytoplasmic body production in the Sua; (2) vesiculation at the tip of the cytoplasmic projection of the Svn; and (3) vesicle-containing body accumulation in the periplasm of both the Svn and Sua.
    Type of Medium: Electronic Resource
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