ISSN:
1433-2981
Keywords:
Immunodeficiency
;
Intracellular calcium
;
Myeloperoxidase
;
Oxidative metabolism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Neutrophils from young calves (2-4 months old) had significantly lower iodination, oxidative metabolism, chemotaxis and random migration, but higher antibody-independent cell-mediated cytotoxicity (AINC) activity than neutrophils from mature cattle (2–3-years old). Antibody-dependent cell-mediated cytotoxicity (ADCC) and oxidative metabolism were enhanced, and random migration was diminished in both groups by in vitro recombinant bovine IFN-γ (rboIFN-γ) treatment. Neutrophils from both groups had enhanced AINC activity and reduced chemotactic responsiveness after incubation in rboIFN-γ, however, these differences were significant only in the mature cattle. RboIFN-γ decreased the cytoplasmic Ca2+ flux in both groups. Neutrophils from young calves had lower cytochrome c reduction and iodination activity than mature cattle in response to both phorbol myristate acetate and opsonised zymosan. Lysed neutrophils from young calves had significantly less myeloperoxidase activity than those from mature cattle. It was also observed that the size of neutrophils increased with age in calves.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00426167
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