ISSN:
1573-5001
Keywords:
3D NMR
;
J coupling constants
;
Isotopic labeling
;
Protein structure
;
χ1 angle
;
SOFT-HCCH-COSY
;
SOFT-HCCH-E.COSY
;
Selective heteronuclear decoupling
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary A sensitive method to assign Hβ protons stereospecifically as well as to determine rotamer populations about χ1, in two 3D experiments is presented. The SOFT-HCCH-COSY experiment allowed us to measure the3J(Hβ,C′) couplings, using constant time evolution of Cα in t2 and Caliphatic-selective decoupling during t3. The SOFT-HCCH-E.COSY experiment allowed us to measure the3J(Hα,Hβ) couplings, using constant time evolution of Cα in t2, a small flip angle1H excitation pulse in the second mixing time, and double-band-selective decoupling (aliphatic and carbonyl carbons) during t3. The method was applied to ribonuclease T1.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02192847
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