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  • 1990-1994  (4)
  • Louisiana  (2)
  • fluid secretion  (2)
  • coupled transport
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Environmental management 18 (1994), S. 271-282 
    ISSN: 1432-1009
    Keywords: Wetland ; Restoration ; Dredging ; Spoil ; Louisiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The rationale and outline of an implementation plan for restoring coastal wetlands in Louisiana is presented. The rationale for the plan is based on reversing the consequences of documented cause-and-effect relationships between wetland loss and hydrologic change. The main feature is to modify the extensive interlocking network of dredged spoil deposits, or spoil banks, by reestablishing a more natural water flow at moderate flow velocity (〈5 cm/sec). Guidelines for site selection from thousands of potential sites are proposed. Examples of suitable sites are given for intermediate marshes. These sites exhibit rapid deterioration following partial or complete hydrologic impoundment, implying a strong hydrologic, rather than sedimentological, cause of wetland deterioration. We used an exploratory hydrologic model to guide determination of the amount of spoil bank to be removed. The results from an economic model indicated a very effective cost-benefit ratio. Both models and practical experience with other types of restoration plans, in Louisiana and elsewhere, exhibit an economy of scale, wherein larger projects are more cost effective than smaller projects. However, in contrast to these other projects, spoil bank management may be 100 to 1000 times more cost effective and useful in wetland tracts 〈1000 ha in size. Modest spoil bank management at numerous small wetland sites appears to offer substantial positive attributes compared to alternative and more intensive management at a few larger wetland sites.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 113 (1990), S. 203-210 
    ISSN: 1432-1424
    Keywords: loop diuretics ; exocrine gland ; fluid secretion ; parotid ; acinar cell ; ion transport ; chloride secretion ; detergent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary We demonstrate that the high affinity bumetanide binding site of the rabbit parotid acinar cell can be extracted from a basolateral membrane fraction using relatively low concentrations (0.07%, wt/vol; 1 mg membrane protein/ml) of the nonionic detergent Triton X-100. This extracted site cannot be sedimented by ultracentrifugation at 100,000 ×g × 1 hr. Bumetanide binding to this site retains the ionic characteristics of bumetanide binding to native membranes but shows a fivefold increase in binding affinity (K d=0.57±0.15 μm vs.K d=3.3±0.7 μm for native membranes). Inactivation of the extracted bumetanide binding site observed at detergent/protein ratios〉1 can be prevented or (partially) reversed by the addition of exogenous lipid (0.2% soybean phosphatidylcholine). When the 0.07% Triton extract is fractionated by sucrose density gradient centrifugation in 0.24% Triton X-100, 0.2% exogenous lipid and 200mm salt, the high affinity bumetanide binding site sediments as a single band withS 20,w =8.8±0.8 S. This corresponds to a molecular weight ∼200 kDa for the bumetanide binding protein-detergent-lipid complex and represents a sevenfold purification of this site relative to the starting membrane fraction. In contrast to previous attempts to purify Na/K/Cl cotransport proteins and their associated bumetanide binding sites, the present method avoids harsh detergent treatment as well as direct covalent modification (inactivation) of the transporter itself. As a consequence, one can follow the still active protein through a series of extraction and purification steps by directly monitoring its bumetanide binding properties.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 120 (1991), S. 125-130 
    ISSN: 1432-1424
    Keywords: loop diuretics ; exocrine gland ; fluid secretion ; lipid ; acinar cell ; ion transport ; chloride secretion ; detergent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary It was recently reported (Turner, R.J., George, J.N., 1990,J. Membrane Biol. 113:203–210) that the high affinity bumetanide binding site of the rabbit parotid Na/K/Cl cotransporter could be extracted from a basolateral membrane preparation from this gland using relatively low concentrations of the non-ionic detergent Triton X-100. At the detergent: protein ratios required for complete membrane solubilization bumetanide binding activity in this extract was lost but could be recovered by the addition of crude soybean lipids. In the present paper the ability of various purified lipids to restore high affinity bumetanide binding activity in detergent solubilized rabbit parotid basolateral membranes is studied. We show that the effect of exogenous lipid on the detergent-inactivated bumetanide binding site is to increase the affinity of binding without affecting the number of binding sites. Of the 11 lipid species tested, several relatively minor, negatively charged membrane phospholipids are the most effective in restoring binding activity (phosphatidylserine ≈ phosphatidylglycerol 〉 phosphatidylinositol 〉 cardiolipin). while the major mammalian plasma membrane lipid components phosphatidylcholine, phosphatidylethanolamine, sphingomyelin and cholesterol are without effect. In addition, we show that in the presence of these minor lipids the affinity of bumetanide binding is considerably increased over that observed in the native membrane (e.g.,K d ≈0.06 μm in membranes extracted with 0.3% Triton and treated with 0.15% wt/vol phosphatidylserine,vs. K d ≈3 μm in native basolateral membranes). This dramatic dependence of bumetanide binding affinity on the presence of certain lipid species suggests that the properties of the bumetanide binding proteinin situ may be quite dependent on the minor lipid content of the plasma membrane. This effect may account for the relatively large variations in bumetanide binding affinity observed from tissue to tissue.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Wetlands ecology and management 3 (1994), S. 63-78 
    ISSN: 1572-9834
    Keywords: Saltmarsh ; Spartina alterniflora ; restoration ; Louisiana ; mitigation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wetland restoration is largely a developing science and engineering enterprise. Analyses of results are too few and constrained to observations over a few years. We report here on the effectiveness of one restoration technique used sparsely in coastal Louisiana for several decades. Canals have been dredged in coastal Louisiana wetlands since 1938 for oil and gas exploration and extraction. These canals are typically dredged to 2.5 m depth and are 20 to 40 m wide. Canal lengths vary from 100 m to several 1000s m in the case of outer continental shelf pipeline canals that cross the wetlands. Today, thousands of miles of canals crisscross these wetlands. Studies have linked dredged canals to a number of undesirable effects on the wetland environment including alterations in salinity, flooding and drainage patterns, direct loss of marsh by convention to open water, and increases in marsh erosion rates. These effects have led state and federal agencies charged with managing the wetland resource to look for methods of mitigating canal impacts. One possible method of managing spoil banks after the abandonment of a drilling site is to return spoil material from the spoil banks to the canal with the hope that marsh vegetation will be reestablished on the old spoil banks and in the canal. The movement of former spoil bank material back into the canal is referred to as ‘backfilling’. The purpose of this study was to (1) examine how backfilled canals changed over 10 years, (2) examine factors influencing success with multiple regression statistical models, and, (3) compare costs of backfilling with other Louisiana marsh restoartion projects. We examined the sites to document and interpret changes occurring since 1983/4 and to statistically model the combined data derived from these new and previous analyses. Specifically, we wanted to determine the recovery rates of vegetation, water depth, and soils in backfilled canals, ‘restored’ spoil banks, and in nearby marshes, and to quantify the influence of plugging canals on these rates. The major factors determining backfilling restoration success are the depth of the canal, soil type, canal dimensions, locale, dredge operator skill, and permitting conditions. Plugging the canal has no apparent effect on water depth or vegetation cover, with the exception that submerged aquatic vegetation may be more frequently observed behind backfilled canals with plugs than in backfilled canals without plugs. Canal age, soil organic matter content, and whether restoration was done as mitigation on-site or off-site were the most important predictors of final canal depth. Canal length and percentage of spoil returned (+) had the greatest effect on the restoration of vegetation cover. Backfilled canals were shallower if they were older, in soils lower in organic matter, and backfilled off-site. Backfilling the canal restores wetlands at a cost of $1,200 to $3,400/ha, which compares very favorably with planned restoration projects in south Louisiana.
    Type of Medium: Electronic Resource
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