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Digitale Medien

Normal-phase HPLC quantitation of chlorophyll a′ and phylloquinone in Photosystem I particles (1993)

Maeda, H. ; Watanabe, T. ; Kobayashi, S. ; [weitere]

Springer

Photosynthesis research 35 (1993), S. 179-184

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ISSN: 1573-5079

Schlagwort(e): chlorophyll a′ ; phylloquinone ; Photosystem I ; high-performance liquid chromatography

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Fractionated Photosystem (PS) I particles consisting of six, five or two core proteins were analyzed by HPLC for chlorophyll (Chl) a′ and phylloquinone (PhQ). Each particle had a Chl a/P700 molar ratio of 50–55 and contained ca. 2 molecules of Chl a′ per P700. Deliberate control of eluent composition led to isolated elution of PhQ and β-carotene in the normal-phase chromatogram. Based on these a simple HPLC procedure has been established to determine the PhQ/P700 molar ratio, which was ca. 2 for the larger two PS I particles and ca. 1 for the smallest particle, in line with previous reports.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00014748

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