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  • 1
    ISSN: 1432-0983
    Keywords: Polyadenylation ; RNA 3′-end formation ; Transcription termination ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have tested the functioning of the human c-myc polyadenylation signal in Saccharomyces cerevisiae. A DNA fragment containing the two AATAAA polyadenylation signals of the c-myc gene was inserted into a plasmid designed for the in-vivo testing of polyadenylation signals in yeast. The c-myc fragment had a partial capacity for directing mRNA 3′-end formation in yeast. The 3′-endpoints were 50–100 bp distant from the mRNA 3′-ends mapped in humans. This human DNA fragment is therefore unspecifically functional in yeast, indicating that other sequence elements than the human polyadenylation signal, AATAAA, are necessary for 3′-end formation.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Psychometrika 59 (1994), S. 177-192 
    ISSN: 1860-0980
    Keywords: Partial credit model ; Rasch model ; item response theory ; measurement of change ; assessment of treatment effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Psychology
    Notes: Abstract The partial credit model is considered under the assumption of a certain linear decomposition of the item × category parametersδ ih into “basic parameters”α j. This model is referred to as the “linear partial credit model”. A conditional maximum likelihood algorithm for estimation of theα j is presented, based on (a) recurrences for the combinatorial functions involved, and (b) using a “quasi-Newton” approach, the so-called Broyden-Fletcher-Goldfarb-Shanno (BFGS) method; (a) guarantees numerically stable results, (b) avoids the direct computation of the Hesse matrix, yet produces a sequence of certain positive definite matricesB k ,k=1, 2, ..., converging to the asymptotic variance-covariance matrix of the $$\hat \alpha _j $$ . The practicality of these numerical methods is demonstrated both by means of simulations and of an empirical application to the measurement of treatment effects in patients with psychosomatic disorders.
    Type of Medium: Electronic Resource
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