ISSN:
1420-9071
Keywords:
Crustacean
;
prawn
;
hepatopancreas
;
cell suspension
;
protein synthesis
;
α-amylase activity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract In crustaceans, all the steps in the assimilation of food take place in the hepatopancreas. To facilitate the study of this organ, a method for the dissociation of cell types was developed. The hepatopancreas of the prawnPalaemon serratus was mechanically dissociated and the cells separated by Percoll density-gradient centrifugation. The E and R cells had similar densities of around 1.05 g/ml. The F cells were separated into two distinct fractions with densities of 1.075 and 1.082 g/ml. The B cells sedimented at a density of 1.12 g/ml. The ratio between the two populations of F cells was found to vary during the intermolt cycle while B cells disappeared after the molt. When the density gradient fractions were incubated with3H-leucine, incorporation was highest in the F cell fractions. Measurements of α-amylase activity, indicated that the two populations of F cells may be derived from the same cell type.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01930474
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