ISSN:
1612-1112
Keywords:
Reversed phase TLC
;
Bovine serum albumin
;
Dansyl DL, D, and L amino acids
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The chromatographic behavior of twelve dansyl DL amino acids, one D isomer and eleven L isomers on RP18W/UV254, RP18W/F254s, and Sil C18−50 UV254 plates developed with aqueous—organic solutions containing bovine serum albumin (BSA) as chiral complexing agent has been extensively investigated. Enantiomeric resolution is highly dependent on mobile phase pH and ionic strength, and on the concentration of both BSA and organic modifier. All the racemates have been resolved within a development time of 1 h 30 min. The selectivity factors (α) for the dansyl amino acids have been compared with those from planar chromatography for the corresponding DNP, DNPy, and Fmoc amino acids, and with those of the same dansyl derivatives on a column prepared from BSA bound to silica gel.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02263881
Permalink
