ISSN:
1573-4919
Keywords:
myosin heavy chains
;
myosin genes
;
rat cardiocytes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Summary Serial deletion constructs derived from the 5′-flanking regions of the human cardiac α- and β-myosin heavy chain genes were generated by polymerase chain reaction (PCR) amplifications. Generation of different length chimeric constructs were based on the complete sequence of the human cardiac myosin heavy chain genes [1, 2]. The primers were synthesized with HindIII and BamH1 sites and were linked to any designed nucleotide of the 5′ flanking sequence of the myosin heavy chain gene(s). Following the PCR amplification and the site-directed mutagenesis, the PCR products were verified by DNA sequencing and subsequently ligated to the chloramphenical acetyltransferase (pBLCAT3) reporter gene which was restricted with Hind III and BamH1. Neonatal rat cardiocytes were used to assay the promotor activity (i.e. CAT activity) of different lengths of the chimeric constructs of the gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01096384
Permalink
