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  • 1990-1994  (3)
  • 1
    Digitale Medien
    Digitale Medien
    In vitro selection of optimal DNA substrates for ligation by a water-soluble carbodiimide (1994)
    Springer
    Journal of molecular evolution 38 (1994), S. 558-560 
    Details
    ISSN: 1432-1432
    Schlagwort(e): In vitro selection ; Chemical ligation ; Water-soluble carbodiimide
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have used in vitro selection to investigate the sequence requirements for efficient template-directed ligation of oligonucleotides at 0°C using a water-soluble carbodiimide as condensing agent. We find that only 2 by at each side of the ligation junction are needed. We also studied chemical ligation of substrate ensembles that we have previously selected as optimal for ligation by RNA ligase or by DNA ligase. As anticipated, we find that substrates selected with DNA ligase ligate efficiently with a chemical ligating agent, and vice versa. Substrates selected using RNA ligase are not ligated by the chemical condensing agent and vice versa. The implications of these results for prebiotic chemistry are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00175874
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  • 2
    Digitale Medien
    Digitale Medien
    The cyclization of arabinosyladenine-5′-phosphorimidazolide (1991)
    Springer
    Journal of molecular evolution 32 (1991), S. 358-359 
    Details
    ISSN: 1432-1432
    Schlagwort(e): Template-directed oligomerization ; Ribosyl nucleosides ; Arabinosyl nucleosides ; Cyclization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary When arabinosyladenine-5′-phosphorimidazolide is allowed to decompose in aqueous solution at room temperature and pH 7.2, depending on the buffer, 5–24% is converted to the 2′,5′-cyclic phosphate (V). Although the extent of cyclization is much greater than for adenosine-5′-phosphorimidazolide, cyclization is less efficient than hydrolysis and so would not substantially decrease the efficiency of condensation reactions in aqueous solution. The significance of this result for prebiotic chemistry is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02101274
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  • 3
    Digitale Medien
    Digitale Medien
    Template-directed oligomerization of 5′-deoxy-5′-nucleosideacetic acid derivatives (1990)
    Springer
    Origins of life and evolution of the biospheres 20 (1990), S. 151-160 
    Details
    ISSN: 1573-0875
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie
    Notizen: Abstract 5′-Deoxy-5′-nucleosideacetic acids II–V are isostructural analogues of nucleotides with a carboxylate group in the place of the 5′-phosphate group. We have studied their oligomerization in aqueous solution using a water-soluble carbodiimide as the condensing agent in the presence or absence of an appropriate polynucleotide template. Condensation of adenylic acid analogues IIa, IIIa, and Va in the presence of polyuridylic acid were found to be the most efficient reactions. Cyclization of the activated monomers to lactones and the insolubility of the oligomers in aqueous solution were found to be obstacles to the efficient formation of long oligomers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01808276
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