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  • 1
    Electronic Resource
    Electronic Resource
    Cloning, sequencing, and characterization of multicopy suppressors of a mukB mutation in Escherichia coli (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 13 (1994), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The mukB gene codes for a 177kDa protein, which might be a candidate for a force-generating enzyme in chromosome positioning in Escherichia coli. The mukB106 mutant produces normal-sized, anucleate cells and shows a temperature-sensitive colony formation. To Identify proteins interacting with the MukB protein, we isolated three multicopy suppressors (msmA, msmB, and msmC) to the temperature-sensitive colony formation of the mukB106 mutation. The msmA gene, which could not suppress the production of anucleate cells, was found to be identical to the dksA gene. The msmB and msmC genes suppressed the production of anucleate cells as well as the temperature-sensitive colony formation. However, none of them couid suppress both phenotypes in a mukB null mutation. DNA sequencing revealed that the msmB gene was identicai to the cspC gene and that the msmC gene had not been described before. A homology search revealed that the amino acid sequences of both MsmB and MsmC possessed high similarity to proteins containing the cold-shock domain, such as CspA of E. coliand the Y-box binding proteins of eukaryotes; this suggests that MsmB and MsmC might be DNA-binding proteins that recognize the CCAAT sequence. Hence, the msmB and msmC genes were renamed cspC and cspE, respectively. Possible mechanisms for suppression of the mukB106 mutation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1994.tb00424.x
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  • 2
    Electronic Resource
    Electronic Resource
    Two mutant alleles of mukB, a gene essential for chromosome partition in Escherichia coli (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 123 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The MukB protein is essential for chromosome partitioning in Escherichia coli and consists of 1484 amino acid residues (170 kDa). We have determined the base changes at the mutated sites of the mukB106 mutant and a newly isolated mutant, mukB33. These mutant mukB genes were each found to carry a single base-pair transition which leads to an amino acid substitution; a serine residue at position 33 was changed to phenylalanine in the case of mukB106, and an aspartic acid residue at position 1201 was changed to asparagine in the case of mukB33.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07196.x
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of translucent segments observed in an smbA mutant of Escherichia coli (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 116 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The smbA gene of Escherichia coli is essential for cell proliferation. The smbA2 mutant shows cold-sensitive colony formation at 22°C. A novel morphological phenotype, formation of a translucent segment at midcell or at a cell pole, was observed by phase-contrastt microscopy at a high frequency in the smbA2 mutant cells incubated in L medium lacking NaCl at 22°C, but not observed in L medium containing 1% NaCl or 20% sucrose at the same temperature. No translucent segment was observed in the wild-type cells in any of the media used. Electron microscopic observation revealed that the translucent segments resulted from the enlargement of a periplasmic space by separation of the inner membrane from the peptidoglycan layer and the outer membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06676.x
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  • 4
    Electronic Resource
    Electronic Resource
    New killing system controlled by two genes located immediately upstream of the mukB gene in Escherichia coli (1994)
    Springer
    Molecular genetics and genomics 243 (1994), S. 136-147 
    Details
    ISSN: 1617-4623
    Keywords: Escherichia coli ; Plasmid Post-segregational killing system ; kicA ; kicB
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nucleotide sequence was determined of the region upstream of the mukB gene of Escherichia coli. Two new genes were found, designated kicA and kicB (killing of cell); the gene order is kicB-kicA-mukB. Promoter activities were detected in the regions immediately upstream of kicB and kicA, but not in front of mukB. Gene disruption experiments revealed that the kicA disruptant was nonviable, but the kicB-disrupted mutant and the mutant lacking both the kicB and kicA genes were able to grow. When kicA disruptant cells bearing a temperature-sensitive replication plasmid carrying the kicA + gene were grown at 30° C and then transferred to 42° C, the mutant cells gradually lost colony-forming ability, even in the presence of a mukB + plasmid. Rates of protein synthesis, but not of RNA or DNA synthesis, fell dramatically during incubation at 42° C. These results suggested that the kicB gene encodes a killing factor and the kicA gene codes for a protein that suppresses the killing function of the kicB gene product. It was also demonstrated that KicA and KicB can function as a post-segregational killing system, when the genes are transferred from the E. coli chromosome onto a plasmid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00280310
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  • 5
    Electronic Resource
    Electronic Resource
    Linear multimer formation of plasmid DNA in Escherichia coli hopE (recD) mutants (1990)
    Springer
    Molecular genetics and genomics 224 (1990), S. 1-9 
    Details
    ISSN: 1617-4623
    Keywords: Rolling circle replication ; Linear plasmid multimers ; recD gene ; hop mutants ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The hopE mutants of Escherichia coli, which cannot stably maintain a mini-F plasmid during cell division, have mutations in the recD gene coding for subunit D of the RecBCD enzyme (exonuclease V). A large amount of linear multimer DNA of mini-F and pBR322 plasmids accumulates in these hopE mutants. The linear multimers of plasmid DNA in the hopE (recD) mutants accumulate in sbc + genetic backgrounds and this depends on the recA + gene function. Linear plasmid multimers also accumulated in a recBC xthA triple mutant, but not an isogenic xthA mutant or an isogenic recBC mutant. The recBC xthA mutant is defective in the conjugative type of recombination. Linear plasmid multimers were not detected in the recBC strain. We propose models to account for linear multimer formation of plasmids in various mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259444
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  • 6
    Electronic Resource
    Electronic Resource
    Multicopy suppresors, mssA and mssB, of an smbA mutation of Escherichia coli (1994)
    Springer
    Molecular genetics and genomics 243 (1994), S. 9-16 
    Details
    ISSN: 1617-4623
    Keywords: smbA ; Aspartokinase ; Gene expression ; NMP kinase ; RNA helicase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated and characterized two multicopy suppressors, mssA and mssB, which suppress the cold-sensitive growth phenotype of the smbA2 mutant of Escherichia coli. The mssA gene is located immediately upstream of the rpsA gene (20.5 min). MssA protein was found to be related to nucleoside monophosphate kinases. The mssB, gene was found to be identical to the deaD gene (69 min), which encodes a putative RNA helicase. The SmbA protein belongs to the aspartokinase family and probably represents a new, fourth aspartokinase species in E. coli. Expression of the smbA gene is essential for cell growth. The smbA2 mutant shows a pleiotropic phenotype characterized by cold-sensitive growth, hypersensitivity to the detergent sodium dodecyl sulfate, and formation of a translucent segment at midcell or at a pole of the cell when grown at 22° C. In addition, some cellular proteins were either increased or decreased in amount in the smbA2 mutant. SmbA may be a regulatory factor in the expression of a battery of genes. MssA and MssB might also relate to the expression of some of these genes. Multiple copies mssA and mssB, suppressed the various phenotypic features of the smbA2 mutant to various extents, suppressing the cold-sensitive growth completely.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00283870
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  • 7
    Electronic Resource
    Electronic Resource
    Maintenance of plasmids in HU and 1HF mutants of Escherichia coli (1990)
    Springer
    Molecular genetics and genomics 220 (1990), S. 197-203 
    Details
    ISSN: 1617-4623
    Keywords: Mini-P1 plasmid ; Mini-F plasmid ; Replication ; hupA-hupB mutant ; himA-hip mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Complementation and sequencing analyses revealed that the hopD mutants, which could not support stable maintenance of mini-F plasmids (Niki et al. 1988), had mutations in the hupB gene, and that the hopD410 mutation was an ochre mutation at the 5th Gln position of HU-1. Maintenance and stability of various plasmids, mini-P1 plasmids, mini-F plasmids, and oriC plasmids, were studied in the hupA and hupB mutants (HU mutants), and himA and hip mutants (IHF mutants). Mini-P1 plasmids and mini-F plasmids could not be introduced into the ΔhupA-ΔhupB double deletion mutant. Replication of mini-F plasmids was partially inhibited in the hupB mutants, including the ΔhupB and hopD(hupB) mutants, whereas replication of oriC plasmids was not significantly affected even in the ΔhupA-ΔhupB double deletion mutant. The mini-P1 plasmid was slightly unstable in the himA-hip mutant, whereas the mini-F plasmid was stable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260482
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  • 8
    Electronic Resource
    Electronic Resource
    Possible involvement of the ugpA gene product in the stable maintenance of mini-F plasmid in Escherichia coli (1990)
    Springer
    Molecular genetics and genomics 223 (1990), S. 361-368 
    Details
    ISSN: 1617-4623
    Keywords: seg-3 mutant ; F plasmid ; Plasmid maintenance ; Heat shock response ; Phosphate transport system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The seg-3 mutant Escherichia coli does not support the maintenance of mini-F plasmid at 42° C. We cloned the chromosomal DNA segment of the wild-type strain W3110 that complements the Seg− phenotype of this mutant. Cleavage mapping of this segment showed that it was derived from the 76-min region of the E. coli chromosome map. Complementation tests using plasmids carrying subcloned DNA segments suggested that the seg-3 mutant carried two mutations that additively affected the maintenance of mini-F plasmid; one was in the ugpA gene and the other was presumably in the rpoH gene. We generated a disrupted ugpA null mutant and found that the mini-F plasmid was unstable in this ugpA null mutant even at 30° C. This suggests that the ugpA gene product is required for the stable maintenance of mini-F plasmid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264441
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