ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The Eu3+ ion induces a B to Z transition in poly(dG-dC) · poly(dG-dC) and poly(dG-m5dC) · poly(dG-m5dC). Other conformations were also elicited by this ion. The conformational changes of these polynucleotides were followed by CD and uv spectroscopy. Eu3+ excitation spectra and Eu3+ excited-state lifetimes were measured for the Eu3+ ion bound to the duplexes in various conformations. B-form poly(dG-dC) and poly(dG-m5dC) appear to have a single class of the Eu3+ binding site while the Z-form observed conformation exhibits two. The numbers of H2O molecules coordinated to the Eu3+ ion bound to the various conformations of the duplexes were determined by measuring Eu3+ excited-state lifetimes in H2O and D2O. The first-order rate constants for the Eu3- and [Co(NH3)6]3+ induced B to Z transitions were measured. The Eu3+ mediated conversion is much faster than the [Co(NH3)6]3+ induced conversion in both polynucleotides.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.360300106
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