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  • 1
    Electronic Resource
    Electronic Resource
    “Assessing Public Exposure to Pesticide-Contaminated Ground Water.” by W. E. Kastenberg and H. C. Yeh (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 32 (1994), S. 0 
    Details
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6584.1994.tb00909.x
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  • 2
    Electronic Resource
    Electronic Resource
    The distribution of hydroxyproline-rich glycoprotein mRNAs in shoots of cucumber and pea (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 82 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The distribution of hydroxyproline-rich glycoprotein (HRGP) mRNAs in the shoots of dark-grown and irradiated cucumber (Cucumis sativus L. cv. Burpee pickler) and pea (Pisum sativum L. cv. Alaska) was studied. A cloned genomic DNA fragment encoding carrot (Daucus carota) root extensin (pDC5A1) was used to measure HRGP mRNAs from cucumber and pea along the length of dark-grown and irradiated shoots. There was a marked difference in the levels of HRGP mRNAs isolated from apical and basal regions of cucumber. Whereas apical, elongating regions had low levels of HRGP mRNAs, basal regions of the shoot had high levels. Levels of HRGP mRNAs were also compared in shoots of dark-grown and irradiated cucumber. Although light inhibits hypocotyl growth, it had no effect on levels of HRGP mRNAs. There was no gradient in the distribution of HRGP mRNAs along the epicotyl of dark-grown pea. As was the case with cucumber, light did not affect the accumulation of HRGP mRNAs in pea shoots. We conclude that light does not affect elongation by regulating the accumulation of HRGP mRNAs. The gradient of accumulation of HRGP mRNAs along the hypocotyl of cucumber probably reflects differences in cellular differentiation along the shoot.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb02951.x
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  • 3
    Electronic Resource
    Electronic Resource
    Using Soil and Meteorologic Data Bases in Unsaturated Zone Modeling of Pesticides (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water monitoring & remediation 10 (1990), S. 0 
    Details
    ISSN: 1745-6592
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Various types of models are being used to evaluate pesticide transport and transformation in the unsaturated zone. Model predictions can be used, for example, to develop alternative agricultural management strategies for pesticide use. However, intensive data requirements for transient models sometimes deter their use. Site-specific measurements are preferred, but existing data bases can be used as a source of required model parameters, especially weather and soil characteristics. These existing data bases make possible the use of models to predict leaching potential in a wide variety of environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6592.1990.tb00026.x
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  • 4
    Electronic Resource
    Electronic Resource
    The expression of barley α-amylase genes inXenopus laevis oocytes (1990)
    Springer
    Planta 180 (1990), S. 333-340 
    Details
    ISSN: 1432-2048
    Keywords: Aleurone ; α-Amylase gene expression ; Gene expression (Xenopus oocytes) ; Hordeum (α-amylase genes) ; cDNA clones (α-amylase) ; Xenopus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The synthesis and secretion of α-amylase (EC 3.2.1.1) fromXenopus laevis oocytes injected with plasmids containing barley α-amylase complementary DNA (cDNA), genomic DNA, or synthetic α-amylase mRNA were studied. α-Amylase accumulated within the oocytes beginning 12 h after injection of DNA and in the medium 12 h later as a result of secretion. S1 mapping showed that the transcription of genomic DNA was initiated at the same site in oocytes as in barley aleurone, but that the transcription of cDNAs was less precise than that of genomic DNA. The α-amylase secreted by oocytes injected with either RNA or DNA had a molecular mass (Mr) of 44000 daltons (Da) and was indistinguishable from native barley α-amylase in size, isoelectric point, antigenicity and enzymatic activity. Isoelectric focussing showed that two enzymatically active isoforms of α-amylase were synthesized and secreted from oocytes injected with synthetic RNA or DNA. The results permit us to assign specific electrophoretic bands to specific cDNA clones. We conclude that theXenopus oocyte is a promising surrogate system for the study of transcription and translation of plant genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01160388
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  • 5
    Electronic Resource
    Electronic Resource
    Hormonal regulation of Ca2+ transport in the endomembrane system of the barley aleurone (1993)
    Springer
    Planta 189 (1993), S. 507-515 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Aleurone ; Calcium transport ; Endomembrane system ; Gibberellin ; Hordeum (Ca2+transport)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gibberellic acid (GA3) and abscisic acid (ABA) regulate α-amylase production in aleurone tissue of the barley (Hordeum vulgare L.) grain during germination. Since α-amylase is a Ca2+-containing protein that is synthesized on the rough endoplasmic reticulum (ER), we investigated the possibility that GA3 and ABA regulate the flux of Ca2+ into the ER of aleurone cells. Membrane vesicles were isolated from GA3- and ABA-treated aleurone layers to characterize the effect of these hormones on calcium transport (CaT) activity as measured by the uptake of 45CaCl2 in vitro. We found that ABA and GA3 greatly alter the relative amounts of CaT activity in ER and tonoplast (TP) membrane fractions. Treatment with GA3 results in CaT being associated primarily with the ER, whereas treatment with ABA results in roughly equal amounts of CaT in ER and TP. Moreover, we found that ABA prevents or reverses the GA3-induced increase in CaT in the ER. The effects of ABA and GA3 on CaT in the ER require several hours to develop, and the effects of these hormones on CaT cannot be brought about by adding the hormones to membranes in vitro. In order to determine whether the in-vitro measurements of CaT reflect changes that occur in vivo we measured the Ca2+ content of isolated ER, measured Ca2+ transport into aleurone layers, and examined differences in the ability of ER proteins from GA- and ABA-treated layers to bind Ca2+. Our results indicate that GA3 increases and ABA decreases both Ca2+ flux into the ER and the amount of calcium that accumulates in the ER of barley aleurone cells in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198213
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  • 6
    Electronic Resource
    Electronic Resource
    Calmodulin stimulation of unidirectional calcium uptake by the endoplasmic reticulum of barley aleurone (1993)
    Springer
    Planta 190 (1993), S. 289-296 
    Details
    ISSN: 1432-2048
    Keywords: Aleurone ; Calcium uptake ; Calmodulin ; Endoplasmic reticulum ; Gibberellic acid ; Hordeum ; (Ca2+ uptake)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The role of calmodulin (CaM) in gibberellic acid (GA3)-stimulated Ca2+ uptake was investigated in endomembranes isolated from aleurone cells of barley (Hordeum vulgare L.). Unidirectional Ca2+ -uptake activity of endoplasmic reticulum (ER) was higher in membranes isolated from aleurone layers treated for 16 h with GA3 and Ca2+ compared with those isolated from layers incubated in Ca2+ alone. However, the level of uptake from Ca2+-treated tissue could be stimulated to that of the GA3-treated cells by applying exogenous CaM which increased the V max of the Ca2+ transporter approximately threefold. Calcium uptake in ER from GA3-treated tissue was inhibited by the CaM antagonist W7 in 50% of experiments, whereas the activity in membranes from non-GA3-treated tissue was unaffected. Treatment with GA3 also led to a twofold increase in CaM levels in aleurone layers within 4–6 h, paralleling the time course of the stimulation of Ca2+ uptake and preceding the stimulation of α-amylase secretion. We propose that the elevation of Ca2+ uptake into the ER induced by GA3 may be coordinated and regulated by elevated levels of membrane-associated CaM and this may regulate Ca2+-dependent α-amylase synthesis in the lumen of the ER.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196956
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  • 7
    Electronic Resource
    Electronic Resource
    Editorial (1993)
    Springer
    Planta 190 (1993), S. 431-431 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224779
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  • 8
    Electronic Resource
    Electronic Resource
    The expression of barley α-amylase genes in Xenopus laevis oocytes (1990)
    Springer
    Planta 180 (1990), S. 333-340 
    Details
    ISSN: 1432-2048
    Keywords: Aleurone ; α-Amylase gene expression ; Gene expression (Xenopus oocytes) ; Hordeum (α-amylase genes) ; cDNA clones (α-amylase) ; Xenopus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The synthesis and secretion of α-amylase (EC 3.2.1.1) from Xenopus laevis oocytes injected with plasmids containing barley α-amylase complementary DNA (cDNA), genomic DNA, or synthetic α-amylase mRNA were studied. α-Amylase accumulated within the oocytes beginning 12 h after injection of DNA and in the medium 12 h later as a result of secretion. S1 mapping showed that the transcription of genomic DNA was initiated at the same site in oocytes as in barley aleurone, but that the transcription of cDNAs was less precise than that of genomic DNA. The α-amylase secreted by oocytes injected with either RNA or DNA had a molecular mass (Mr) of 44000 daltons (Da) and was indistinguishable from native barley α-amylase in size, isoelectric point, antigenicity and enzymatic activity. Isoelectric focussing showed that two enzymatically active isoforms of α-amylase were synthesized and secreted from oocytes injected with synthetic RNA or DNA. The results permit us to assign specific electrophoretic bands to specific cDNA clones. We conclude that the Xenopus oocyte is a promising surrogate system for the study of transcription and translation of plant genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198784
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    Paper (German National Licenses)
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