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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 30 (1990), S. 533-536 
    ISSN: 1612-1112
    Keywords: Protein purification ; HPLC of receptor ; Calcium channel ; 1,4-Dihydropyridine receptor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Functionally intact 1,4-dihydropyridine receptors have been isolated from digitonin-solubilized skeletal muscle membranes with the combined use of Wheatgerm Lectin-Sepharose affinity chromatography and ion-exchange HPLC. Lectin affinity chromatography was used for the purification of glycoproteins containing N-acetyl-glucosamines residues. Separation of functionally intact 1,4-dihydropyridine receptor/calcium channel complex from Mg2+-ATPase, one of the most abundant glycoproteins in skeletal muscle T-tubular membranes, was successfully performed by HPLC on a TSKgel DEAE-5PW column.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Metabolexpert® ; HPLC-Metabolexpert® ; Ecdysone ; Metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Computer simulation of ecdysone metabolism in insects has been done by the a software called HPLC-Metabolexpert®, that served to generate the metabolic pathways of ecdysone in a retrospective manner. Some of the generated metabolites have already been detected, others are to be confirmed. Lists of the applied metabolic transformations, the predicted metabolites and their HPLC elution times are also given.
    Type of Medium: Electronic Resource
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