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Fat-soluble vitamin concentration in chronic alcohol-induced pancreatitis (1994)

Marotta, F. ; Labadarios, D. ; Frazer, L. ; [et al.]

Springer

Digestive diseases and sciences 39 (1994), S. 993-998

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ISSN: 1573-2568

Keywords: pancreatitis ; steatorrhea ; fat-soluble vitamin

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract In order to study the fat-soluble vitamin concentration of patients with chronic alcohol-induced pancreatitis (CAIP) we measured vitamins A and E, total lipids, and retinol-binding protein (RBP) in the plasma of 44 patients with CAIP and 83 controls (44 healthy controls; 39 Crohn's disease patients). Mean plasma vitamin E and vitamin E/total lipid ratio were significantly lower in CAIP when compared with either control or Crohn's disease groups. A low vitamin E/total lipid ratio was found in 75% of CAIP patients (91% with steatorrhea) and a ratio less than 1.0 was virtually 100% predictive of steatorrhea. The mean plasma vitamin A level for the CAIP group was significantly lower (overall 16%, 38% with steatorrhea) than in controls. Patients with CAIP show subnormal plasma levels vitamin E more often as compared to vitamin A. Further, the plasma vitamin E/total lipids ratio may be a sensitive and practical means in the detection and follow-up of steatorrhea in these patients.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02087550

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Determination of amino acids in the hepatic and brain tissue of the rat by gas chromatography (1991)

Moodie, T. M. ; van der Westhuizen, L. ; Labadarios, D.

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 14 (1991), S. 579-583

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ISSN: 0935-6304

Keywords: Gas chromatography ; Fused silica capillary column ; Cold on-column injection ; Amino acid analysis ; Liver ; Brain ; Rat ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: A procedure is described for the quantitative determination of amino acids in hepatic and brain tissue samples from the rat. Because the presence of certain matrix components in the tissue material led to interference with chromatographic analysis they were removed by a prechromatographic “clean-up” step.Quantitative analysis of amino acids, as their N-heptafluorobutyryl iso-butyl ester derivatives, was achieved by high resolution gas chromatography on an apolar fused silica open tubular column. Reproducibility data from the complete procedure are presented; coefficients of variation for arginine and histidine in hepatic tissue varied between 7.1 and 10.1% whereas those for most other amino acids were better than 5%, with a mean recovery of 90%.

Additional Material: 2 Ill.