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  • 1
    Electronic Resource
    Electronic Resource
    Determination of the monomer-dimer equilibrium of interleukin-8 reveals it is a monomer at physiological concentrations (1994)
    s.l. : American Chemical Society
    Biochemistry 33 (1994), S. 12741-12745 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00209a002
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  • 2
    Electronic Resource
    Electronic Resource
    The Role of the Insulin-like Growth Factor System in Neuronal Rescue (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 692 (1993), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1993.tb26212.x
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  • 3
    Electronic Resource
    Electronic Resource
    The mycorrhizal status of Welwitschia mirabilis (1993)
    Springer
    Mycorrhiza 3 (1993), S. 13-17 
    Details
    ISSN: 1432-1890
    Keywords: Gnetales ; Welwitschia mirabilis ; Vesicular-arbuscular mycorrhizae ; Namib Desert
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This is the first report of the mycorrhizal status of Welwitschia mirabilis, a gymnosperm endemic to the Namib Desert. Like all other gymnosperms except the Pinaceae and Gnetaceae, W. mirabilis is associated with vesicular-arbuscular mycorrhizal (VAM) fungi. Mycorrhizal colonization of roots and the diversity and abundance of VAM species were determined at seven sites. Six sites received annual rainfall of 0–100 mm, varying widely from year to year. The seventh site experienced more predictable annual rainfall of 150–200 mm. Perennial vegetation was sparse at the six low-rainfall sites. Dry annual grasses from previous rain events were present at only three of these six sites and mean mycorrhizal colonization levels of W. mirabilis at these three sites were as high as 18%. W. mirabilis was not mycorrhizal at sites where grasses were absent. The seventh site, receiving higher rainfall, supported small trees and annual grasses in addition to W. mirabilis. Mycorrhizal colonization levels of W. mirabilis at this site were significantly higher than at the other six sites, closely paralleling those of the surrounding annual grasses. The mycorrhizal flora of W. mirabilis consisted of four Glomus species. These taxa were not unique to W. mirabilis, having been found with Stipagrostis and Cladoraphis grasses throughout the Namib and Kalahari deserts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213462
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation of two contigs of overlapping cosmids derived from human chromosomal band 3p21.1 and identification of 5 new 3p21.1 genes (1994)
    Springer
    Somatic cell and molecular genetics 20 (1994), S. 255-265 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Consistent loss of DNA sequences from several regions on the short arm of human chromosome 3 has suggested that multiple tumor suppressor genes reside on chromosome 3p in various types of cancer cells. We have focused our efforts on an analysis of chromosomal band 3p21.1 since aminoacylase-1 (ACY1), which is localized to this band, has been shown to have lower levels of expression in several small cell and non-small cell lung cancer cell lines. Starting with two cosmids within 3p21.1,D3S92 andD3S93, we have isolated two separate contigs of overlapping cosmids within 3p21.1, by screening a library of 5700 chromosome 3-specific cosmid clones. Detailed restriction maps for these two contigs show that they contain multiple clusters of rare cutting restriction endonuclease sites. One contig extends for 100 kb and encompassed bothACY1 andD3S92, and the other extends about 80 kb around theD3S93 locus. Many different restriction fragments derived from these two contigs were found to be evolutionarily conserved and hybridized to distinct message transcripts. These fragments were used to identify homologous cDNAs from an adenogastric cDNA library, and several of these cDNAs were partially sequenced. We have identified five new genes from these two contigs and there is evidence to suggest that several additional genes reside within these cosmid contigs. The genes identified from 3p21.1 were then hybridized to DNA, isolated from a series of lung cancer cell lines and matched normal and tumor DNA from lung cancer patients. No alterations were detected with any of these probes, both at the DNA or RNA levels. A similar analysis with DNA fragments derived from these two genomic regions also failed to detect any alterations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02254716
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  • 5
    Electronic Resource
    Electronic Resource
    Methylation of the 5′ flanking sequences of the ribosomal DNA in human cell lines and in a human-hamster hybird cell line (1992)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 50 (1992), S. 357-362 
    Details
    ISSN: 0730-2312
    Keywords: rDNA ; lymphoblastoid ; methylation ; hypermethylation ; DNA ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: In human lymphoplastoid cell line (Z83) in which rDNA genes on chromosomes 22 are amplified but transcribed at a low level, immunocytological studies with antibodies to 5 methylcytidine provided evidence for hypermethylation of the rDNA. The extent of methylation of the 5′ flanking sequence of the ribosomal DNA was examined by comapring the size of restriciton fragments obtained by digestion of genomic DNA with EcoRI and Hpall or EcoRI and Mpsl. Southern blots indicated hypermethylation of the 5′ flanking sequences of many copies of rRNA genes in these cells, but not in a control lymphoblastoid cell line without rDNA amplification. Results obtained with somatic hybird human-hamster cell line, in which the rRNA genes on the single human chromosomes 22 are inactive, showed that only a small fraction of the CCGG sites in the 5′ flanking sequences of the transcriptionally silent rRNA genes in this hybird were methylated. Since inactive rRNA genes can show such a minimal level of methylation, it is likely that the extreme hypermethylation of the apmlified rRNA genes in Z83 association with their inactivation rather than following it. © 1992 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240500404
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    Paper (German National Licenses)
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