ISSN:
1588-2780
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Energy, Environment Protection, Nuclear Power Engineering
Notes:
Abstract A method is described to separate trace amounts of selenium in biological samples without using a carrier. This method is based on the adsorption on active carbon of the complex ion formed with APDC /ammonium salt of l-pyrrolidine carbodithioic acid/ at pH 1. The efficiency of the radiochemical separation described is measured by using carrier-free75Se labelled solutions of sodium selenite at selenium concentrations from 3.5×10−8 to 3.5×10−11 g ml−1. The results were between 95% and 98% with statistical variations from 2% to 10%. The determination of selenium can be made following this separation either through75Se in the traditional way, or through77mSe if the separation is performed prior to irradiation. The detection limits on the available conditions were 0.01 ppm for75Se and 0.1 ppm for77mSe. When the analysis is performed through75Se /t=120 d/, the statistical error is notably smaller because the counting time may be considerable, whereas through77mSe/t=17.5 s/it is higher than 20%, depending on the concentration and the available neutron flux. However, the advantages of gaining time and the fact of performing the trace separation from a non radioactive material, make both procedures competitive as useful tools for the research on the function of Se in vertebrates.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02163717
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