Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Scandinavian journal of immunology
33 (1991), S. 0
ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Nickel is the major cause of metal-induced allergic dermatitis. Twelve nickel-specific T cell clones were used to investigate the cellular immune reactions occurring in nickel sensitivity. The selection between the alternative T cell receptors α and βγδ and two alternative Vβ genes (Vβ5 and Vβ8) were studied to see if nickel induces a selective pressure for clones bearing particular genes. Cell surface markers were studied by monoclonal antibodies and flow cytometry. Soluble mediators were measured by an ELISA method.The clones used T cell receptor αβ genes but did not use Vβ5 or Vβ8. They were T helper clones with a primed memory marker (CD3+CD4+CD8−CD45RO+) and carried HLA-DR. None of the clones secreted IL-1α, all of them secreted IL-2 receptor. Four clones secreted IL-lβ, six IL-4 and seven IL-6, the peaks in IL-2Rand IL-6 secretion preceding 1L-4 secretion. The clones helped immunoglobulin synthesis.The clones from late effector phase of the nickel allergic reaction favours the use of T cell receptors αβ genes. Nickel-specific clones were phenotypically indistinguishable but differed in soluble mediators produced.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1991.tb01791.x
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