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  • 1
    Electronic Resource
    Electronic Resource
    The combination of normal phase with reversed phase high performance liquid chromatography for the analysis of asparagine-linked neutral oligosaccharides labelled with p-aminobenzoic ethyl ester (1992)
    New York, NY : Wiley-Blackwell
    Biomedical Chromatography 6 (1992), S. 77-83 
    Details
    ISSN: 0269-3879
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: We have developed a novel approach for the analysis of asparagine-linked neutral oligosaccharides derived from glycoproteins. The oligosaccharides are labelled with p-aminobenzoic ethyl ester and the derivatives are separated on two high performance liquid chromatographic columns, one containing amide-silica and the other containing octadecyl-silica. The elution positions of 39 different ABEE-oligosaccharides on the two columns were plotted on a two-dimensional map. Unique non-overlapping positions of these oligosaccharides demonstrate that this technology would be useful for the identification of Asn-linked oligosaccharides at high sensitivity.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bmc.1130060207
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Gas chromatographic separation of protein amino acids in four minutes (1991)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 14 (1991), S. 751-753 
    Details
    ISSN: 0935-6304
    Keywords: Capillary gas chromatography ; Amino acid analysis in five minutes ; Derivatization with ethyl chloroformate ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A method is presented for analysis of protein amino acids in five minutes, including derivatization. The chemical treatment involves reaction with ethyl chloroformate: if the composition of the reaction medium is optimum this reagent esterifies carboxylic groups simultaneously with derivatization of other reactive groups and the reaction is complete in seconds. The moderately polar silicone OV-1701 was found to be a convenient liquid phase for separation of the derivatives: a five meter capillary column of internal diameter suitable for routine use was able to separate the amino acids in four minutes. Derivatization of arginine was incomplete and it failed to elute from the column.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240141110
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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