ISSN:
1432-069X
Keywords:
Skin collagen
;
UV effects
;
Hairless mice
;
Collagen solubility
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract UVA- and UVB-induced alterations in dermal collagen were investigated in a murine animal model. Groups of hairless mice were exposed to UVA and UVB for 28 weeks at a dose of 60 J/cm2 three times weekly and 0.06 J/cm2 three times weekly, respectively. Untreated animals were used as controls. Every 4 weeks dorsal skin was examined for quantitative and qualitative changes in dermal collagen. Neither UVA nor UVB caused a significant alteration in total skin collagen content. However, after UVA treatment the ability of skin collagen to be digested by pepsin decreased dramatically (up to 65% of skin collagen remained insoluble after 4 months), whereas exposure to UVB had no significant effect. Furthermore a shift in the ratio of α1(I,III) chains to α2(I) chains was detected after UVA exposure. The amount of type V collagen in mouse skin, as determined by a sensitive ELISA method, was markedly decreased after UVA treatment, but not after UVB treatment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00371578
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