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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 15 (1992), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Stomatal conductance of individual leaves was measured in a maize field, together with leaf water potential, leaf turgor, xylem ABA concentration and leaf ABA concentration in the same leaves. Stomatal conductance showed a tight relationship with xylem ABA, but not with the current leaf water status or with the concentration of ABA in the bulk leaf. The relationship between stomatal conductance and xylem [ABA] was common for variations in xylem [ABA] linked to the decline with time of the soil water reserve, to simultaneous differences between plants grown on compacted, non-compacted and irrigated soil, and to plant-to-plant variability. Therefore, this relationship is unlikely to be fortuitous or due to synchronous variations. These results suggest that increased concentration of ABA in the xylem sap in response to stress can control the gas exchange of plants under field conditions.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 17 (1994), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The existence of relationships between intercepted photo-synthetic photon flux density (PPFD) and growth of individual organs is somewhat controversial. We have tested whether such relationships could account for the natural variability in elongation rates of taproot and secondary roots of sunflower (from 2 to 135 mm d−1), in field and laboratory conditions. Elongation of taproot and secondary roots was recorded daily through windows in the field. A range of PPFD was obtained by following day-to-day natural fluctuation for three contrasting growing periods, and by shading part of the plants under study. A parallel experiment was carried out in a growth chamber with contrasting light intensities and with a 14CO2 labelling experiment. After the two-leaf stage, i.e. when the contribution of photosynthetic carbon became appreciable in root growth, daily root elongation rate was closely linked to the PPFD intercepted from 36 to 12 h before the measurement of root elongation. Curvilinear relationships applied to plants grown in the field as well as in a growth chamber, and to shaded plants as well as to plants subjected to day-to-day changes in intercepted PPFD. For a given intercepted PPFD, the taproot elongated faster than secondary roots, and secondary roots originating near the base of the taproot elongated faster than those originating near the apex. The elongation rate of any secondary root apex was accounted for (r= 0.77) by the ratio of intercepted PPFD to the distance between the apex and the base of the taproot. No relationships between intercepted PPFD and elongation rate were observed before the two-leaf stage, when the CO2 labelling experiment suggests that carbon essentially originates from the seed. Therefore, this study suggests a role for source-sink relations in the distribution of elongation between apices and a role for carbon nutrition in day-to-day variations of root elongation rate. Precise mechanisms explaining this behaviour remain to be investigated.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 16 (1993), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We describe here an integration of hydraulic and chemical signals which control stomatal conductance of plants in drying soil, and suggest that such a system is more likely than control based on chemical signals or water relations alone. The determination of xylem [ABA] and the stomatal response to xylem [ABA] are likely to involve the water flux through the plant. (1) If, as seems likely, the production of a chemical message depends on the root water status (Ψr), it will not depend solely on the soil water potential (Ψs) but also on the flux of water through the soil-plant-atmosphere continuum, to which are linked the difference between Ψr and Ψs. (2) The water flux will also dilute the concentration of the message in the xylem sap. (3) The stomatal sensitivity to the message is increased as leaf water potential falls. Stomatal conductance, which controls the water flux, therefore would be controlled by a water-flux-dependent message, with a water-flux-dependent sensitivity. In such a system, we have to consider a common regulation for stomatal conductance, leaf and root water potentials, water flux and concentration of ABA in the xylem. In order to test this possibility, we have combined equations which describe the generation and effects of chemical signals and classical equations of water flux. When the simulation was run for a variety of conditions, the solution suggested that such common regulation can operate. Simulations suggest that, as well as providing control of stomatal conductance, integration of chemical and hydraulic signalling may also provide a control of leaf water potential and of xylem [ABA], features which are apparent from our experimental data. We conclude that the root message would provide the plant with a means to sense the conditions of water extraction (soil water status and resisance to water flux) on a daily timescale, while the short-term plant response to this message would depend on the evaporative demand.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 16 (1993), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A model of maize stomatal behaviour has been developed, in which stomatal conductance is linked to the concentration of abscisic acid ([ABA]) in the xylem sap, with a sensitivity dependent upon the leaf water potential (Ψ1). It was tested against two alternative hypotheses, namely that stomatal sensitivity to xylem [ABA] would be linked to the leaf-to-air vapour pressure difference (VPD), or to the flux of ABA into the leaf. Stomatal conductance (gs) was studied: (1) in field-grown plants whose xylem [ABA] and Ψ1 depended on soil water status and evaporative demand; (2) in field-grown plants fed with ABA solutions such that xylem [ABA] was artificially raised, thereby decreasing gs and increasing Ψ1 and leaf-to-air VPD; and (3) in ABA-fed detached leaves exposed to varying evaporative demands, but with a constant and high Ψ1. The same relationships between gs, xylem [ABA] and Ψ1, showing lower stomatal sensitivity to [ABA] at high Ψ1, applied whether variations in xylem [ABA] were due to natural increase or to feeding, and whether variations in Ψ1, were due to changes in evaporative demand or to the increased Ψ1 observed in ABA-fed plants. Conversely, neither the leaf-to-air VPD nor the ABA flux into the leaf accounted for the observed changes in stomatal sensitivity to xylem [ABA]. The model, using parameters calculated from previous field data and the detached-leaf data, was tested against the observations of both ABA-fed and droughted plants in the field. It accounted with reasonable accuracy for changes in gs (r2 ranging from 0.77 to 0.81). These results support the view that modelling of stomatal behaviour requires consideration of both chemical and hydraulic aspects of root-to-shoot communication.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 15 (1992), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: During two seasons, ABA concentrations were monitored in roots, leaves and xylem sap of field-grown maize. The water status of soil and plant was also measured. Plants were grown on plots with compacted or non-compacted soil, which were irrigated or remained unwatered. ABA concentration in the xylem sap before dawn and in the roots increases 25-fold and five-fold, respectively, as the soil dried, with a close correlation with the soil water status, but with no clear effect of the soil structure. In contrast to the results of several laboratory experiments, no appreciable increase in xylem [ABA] and reduction in stomatal conductance were observed with dehydration of the part of the root system located in soil upper layers. These responses only occurred when the water reserve of the whole soil profile was close to depletion and the transpiration declined. Xylem [ABA] measured during the day was appreciably higher in the compacted treatment than in non-compacted treatment, unlike that measured before dawn. Since a mechanical message is unlikely to undergo such day-night alterations, we suggest that this was due to a faster decrease in root water potential and water flux in the compacted treatment, linked to the root spatial arrangement. These results raise the possibility that ABA concentration in the xylem sap could be controlled by two coexisting mechanisms: (1) the rate of ABA synthesis in the roots linked to the soil or root water status, as shown in laboratory experiments; (2) the dilution of ABA in the water flow from roots, which could be an overriding mechanism in field conditions. This second mechanism would allow the plant to sense the water flux through the root system.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. Stomatal conductance, leaf water potential, soil water potential and concentration of abscisic acid (ABA) in the xylem sap were measured on maize plants growing in the field, in two treatments with contrasting soil structures. Soil compaction affected the stomatal conductance, but this effect was no longer observed if the soil water potential was increased by irrigation. Differences in leaf water potential did not account for the differences in conductance between treatments. Conversely, the relationship between stomatal conductance and concentration of ABA in the xylem sap was consistent during the experiment. The proposed interpretation is that stomatal conductance was controlled by the root water potential via an ABA message. Control of the stomatal conductance by the leaf water potential or by an effect of mechanical stress on the roots is unlikely.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Irrigation science 12 (1991), S. 145-152 
    ISSN: 1432-1319
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The ability of water balance models based on the concept of Transpirable Soil Water to predict the occurrence of water stress and the need for irrigation was tested for several environmental conditions of the root system, to determine in which conditions errors are likely to be appreciable. The response of evapotranspiration, stomatal conductance and leaf water potential to soil water reserve was studied under three conditions: (i) in pots with maize plants, (ii) in the field with deep soil and the root system placed in favourable conditions, with wheat during a dry year and with maize during four years with contrasting climate, (iii) in the field, with soil compaction which disturbed the maize root system, decreasing its efficiency for water uptake, during four year. (i) In the pot experiment, where the volume of the Transpirable Soil Water (TSW) is well defined, the responses followed the hypothesis of water balance models. (ii) The soil depletion was higher than the calculated TSW during two dry years in the field, because of an appreciable contribution of the non-rooted soil layers to the water balance. As a consequence, evapotranspiration, stomatal conductance and predawn water potential did not decrease over the whole range of soil water reserve. Grain yield was no lower in those years than in the wet years, in spite of the fact that the soil water reserve was depleted. Thus, a water balance based on the TSW would have underestimated in these conditions the ability of plants to withdraw soil water, overestimating the necessity of irrigation. Predawn water potential gave, on the contrary, indications consistent with the responses of the stomatal conductance and the net CO2 assimilation. (iii) The water uptake by plants would have been overestimated in the case of compacted soil. Stomatal conductance was low even for high levels of the soil water reserve, except if the densely rooted top 0.1 m layer of soil was rewatered by irrigation. Water stress could not have been diagnosed in this case from indications of soil water potential or of pre-dawn water potential. These data confirm that some knowledge of the environmental conditions of the root system is necessary to determine if errors made using water balance models are likely to be appreciable, and to know if they lead to an underestimation or overestimation of the risk of water stress.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 159 (1994), S. 245-254 
    ISSN: 1573-5036
    Keywords: pedology ; root penetration ; root system ; soil structure ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The vertical distribution of maize roots was studied in four contrasting soils, (arenosols, luvisols, planosols and vertisols) by using in-situ root mapping on vertical planes. The relationship between root contact frequency and depth was different for each soil, with a relatively low field-to-field variability within each soil type. The general aspect of this relationship did not change appreciably for three years in arenosols, with a low colonization in sandy layers probably being due to mechanical barriers. The relationship was consistently non-monotonic in luvisols and planosols, because of the sparse colonization of sandy layers. In planosols, these layers were traversed by some primary roots, which were essentially clustered in animal burrows. The distribution of root contact frequency was closer to an exponential function in vertisols. In these soils rooting depth and colonization of deep soil layers showed a marked increase during two dry years compared with a wet year. This was probably due to a denser net of shrinkage cracks and slickensides, where roots were essentially located in dry years. These results raise the possibility of modelling the decrease in root distribution with depth using soil information and climatic characteristics.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 140 (1992), S. 291-301 
    ISSN: 1573-5036
    Keywords: hydraulic conductivity ; modelling ; root spatial arrangement ; root-to-shoot communication ; soil resistance ; water transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We have appraised for clumped root systems the widely-accepted view that the resistance to water flux from soil to roots (‘soil resistance’) is low under most field conditions, so that root water potential would closely follow the mean soil water potential. Three root spatial arrangements were studied, simulating either the regular pattern generally assumed in models, or two degrees of root clumping frequently observed in the field. We used a numerical 2-dimensional model of water transfer which assumes a control of evapotranspiration by root signalling. Calculations were carried out at two evaporative demands and for two contrasting soil hydraulic properties. The rate of soil depletion, the timing of the reduction in evapotranspiration and the difference between root water potential and mean soil water potential were all affected by the root spatial arrangement, with a greater effect at high evaporative demand and low soil hydraulic conductivity. Almost all the soil water reserve was available to plants without reduction in evapotranspiration in the regular case, while only a part of it was available in clumped cases. In the regular case, calculated ‘soil resistances’ were similar to those calculated using Newman's (1969) method. Conversely they were higher by up to two orders of magnitude in clumped root spatial arrangements. These results place doubt on the generality of the view that ‘soil resistance’ is low under common field conditions. They are consistent with the results of field experiments, especially with recent data dealing with root-to-shoot communication.
    Type of Medium: Electronic Resource
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