ISSN:
1432-0983
Keywords:
Saccharomyces cerevisiae
;
Mitochondria
;
Cytochrome c oxidase subunit 1
;
RNA processing
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Eighteen nuclear mutants of the yeast Saccharomyces cerevisiae, each disturbed in the biosynthesis of the mitochondrially encoded cytochrome c oxidase subunit 1 (cox 1) and each representing a distinct complementation group, have been examined to identify the level at which COX1 expression is affected. RNA blotting revealed that most have a defect in the processing of COX1 precursor-mRNA; only a few are defective in COX1 transcription and/or pre-mRNA stability. In most RNA-processing mutants, the absence of the COX1 messenger results from a defect in the splicing of one or more COX1 introns. In turn, this defect can be ascribed to a mutation in a nuclear gene which is either directly involved in splicing or else acts indirectly by impairing COX1 translation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00318473
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