ISSN:
1435-1536
Keywords:
Liposomers
;
spreading
;
surface
;
film
;
phospholipase A2 hydrolysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract The kinetics of surface film formation from DOPC small unilamellar liposomes spread at the air-water interface was studied by recording the surface pressure and the surface potential. The rate constants of the surface transformation of perfectly closed vesicles into open surface active structures was detcrmined. It was found that the surface transformation was accelerated by enzymatid hydrolysis. A theoretical approach, describing the coupling of the surface transformation with the catalytic hydrolysis in the scooting mode of bilayered liposomes is developed. Values of the specific activity of hydrolysis of DOPC bilayered vesicles by phospholipase A2 fromVipera berus orientalis were obtained by this method and compared with those previously obtained by the classical pH-stat tirration method.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00652369
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