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  • 1990-1994  (2)
  • 1
    Electronic Resource
    Electronic Resource
    A low-copy-number Sorghum DNA sequence that detects hypervariable EcoRV fragments (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 64-69 
    Details
    ISSN: 1432-2242
    Keywords: Hybridization probe ; Sorghum bicolor ; Fingerprinting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A sorghum genomic DNA clone that hybridized on Southern blots in simple but different patterns to fragments produced by digestion of DNA from the parents of an F2 mapping population was hybridized to EcoRV-digested DNA from 53 accessions. Forty-six different fragment patterns were observed, each comprised of from one to ten bands. Much less variability was detected in EcoRI than EcoRV digests of a selected subset of the accessions. Base-sequence analysis of the clone did not reveal a functional identity for the sequence and the clone does not contain repeated sequences often associated with hypervariable loci. Clones such as this will be especially useful in evaluating germplasm diversity and in identifying the potential parentage of hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226984
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A RFLP linkage map of Sorghum bicolor (L.) Moench (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 139-145 
    Details
    ISSN: 1432-2242
    Keywords: Sorghum ; RFLPs ; Linkage map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A RFLP linkage map of sorghum composed principally of markers detected with sorghum low-copy-number nuclear DNA clones has been constructed. The map spans 1789 cMs and consists of 190 loci grouped into 14 linkage groups. The 10 largest linkage groups consist of from 10 to 24 markers and from 103 to 237 cMs, and the other 4 linkage groups consist of from 2 to 5 markers and from 7 to 62 cMs. The map was derived in Sorghum bicolor ssp. bicolor by analysis of a F2 population composed of 50 plants derived from a cross of IS 3620C, a guinea line, and BTx 623, an agronomically important inbred line derived from a cross between a zera zera (a caudatum-like sorghum) and an established kafir line. The restriction fragment length polymorphism (RFLP) frequency detected in this population using polymerase chain reaction (PCR)-amplifiable low-copy-number sorghum clones and five restriction enzymes was 51%. A minimal estimate of the number of clones that detect duplicate sequences is 11 %. Null alleles occurred at 13% of the mapped RFLP loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225133
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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