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  • 1985-1989  (2)
  • 1975-1979  (1)
  • 1935-1939
  • Tissue culture  (2)
  • DNA levels  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Trees 2 (1988), S. 32-38 
    ISSN: 1432-2285
    Keywords: Auxin ; Cytokinin ; Gymnosperm ; Micropropagation ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Adventitious bud formation was induced on detached cotyledons and on cotyledons attached to excised embryos of Pinus caribaea and Pseudotsuga menziesii. The embryonic tissue was exposed to the cytokinin 6-benzyl amino purine contained within an agar medium. This exposure resulted in the formation of a meristematic zone, involving both epidermal and sub-epidermal cells, and then nodules on the tissue surface. Some of these nodules were induced to differentiate into bud primordia, and thence shoots, following exposure to a combination of auxin (IAA) and cytokinin. Shoots were produced over the entire surface of detached cotyledons of Ps. menziesii but predominantly on the adaxial surfaces of detached cotyledons of Pi. caribaea and the tips and adaxial surfaces of the cotyledons on the entire embryos of both species. Thus, inter-specific differences in the distribution of competent areas for adventitious bud production were detected in embryos.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 263 (1978), S. 75-81 
    ISSN: 1432-069X
    Keywords: Keratinocyte cell culture ; Retinoic acid ; Thymidine incorporation ; DNA levels ; Keratinocytenkultur ; Vitamin A-Säure ; Thymidin-Einbau ; DNS-Konzentrationen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Der Einfluß von Vitamin A-Säure auf Gewebekulturen von Keratinocyten gewonnen von Meerschweinchen-Ohren wurde untersucht. Vitamin A-Säure sowie die Vorbehandlung der Plastik-Petri-Schalen mit einer Kollagen-Suspension erleichterte die Zelladhäsion und das Wachstum. Vitamin A-Säure versursachte eine DNS-Synthese-Welle, die durch den Einbau von 3H-Thymidin und die Bestimmung der cellulären DNS nachgewiesen werden konnte. Diese DNS-Synthese erfolgte 24–45 h nachdem die Keratinocyten angesetzt wurden. Während dieser Zeitspanne resultierte die Vorbehandlung mit Vitamin A-Säure in einer zehnfachen Erhöhung des Einbaus von 3H-Thymidin und zeigte ein komplexes Muster von Gipfeln und Schultern, welche die synchrone S-Phase der Keratinocyten darstellen könnte.
    Notes: Summary The effect of vitamin A acid on the culture of guinea pig ear keratinocytes was investigated. Use of this agent and treatment of the plastic dishes with collagen solution facilitated attachment and growth. Vitamin A acid elicited a wave of DNA synthesis which was studied by incoporation of tritiated thymidine and quantitation of cellular DNA, particularly during the period 24–45 h after plating. During this period, thymidine incorporation into DNA was increased ten-fold by vitamin A acid and showed a complex pattern of peaks and shoulders which may represent the S phase of synchronous keratinocytes.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Trees 2 (1988), S. 32-38 
    ISSN: 1432-2285
    Keywords: Auxin ; Cytokinin ; Gymnosperm ; Micropropagation ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Adventitious bud formation was induced on detached cotyledons and on cotyledons attached to excised embryos ofPinus caribaea andPseudotsuga menziesii. The embryonic tissue was exposed to the cytokinin 6-benzyl amino purine contained within an agar medium. This exposure resulted in the formation of a meristematic zone, involving both epidermal and sub-epidermal cells, and then nodules on the tissue surface. Some of these nodules were induced to differentiate into bud primordia, and thence shoots, following exposure to a combination of auxin (IAA) and cytokinin. Shoots were produced over the entire surface of detached cotyledons ofPs. menziesii but predominantly on the adaxial surfaces of detached cotyledons ofPi. caribaea and the tips and adaxial surfaces of the cotyledons on the entire embryos of both species. Thus, inter-specific differences in the distribution of competent areas for adventitious bud production were detected in embryos.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
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